SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region

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	SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region
	Sun, Yun 1,2; Sun, Li 1; Xing, Ming-qing 3; Liu, Chun-sheng 4; Hu, Yong-hua 1
刊名	ACTA VETERINARIA SCANDINAVICA
	2013-11-12
卷号	55 页码:78
关键词	Streptococcus iniae DNA vaccine Immune response Virulence
ISSN号	0044-605X
通讯作者	Hu, YH
中文摘要	Background: Streptococcus iniae is a Gram-positive bacterium and a severe pathogen of a wide range of farmed fish. S. iniae possesses a virulence-associated streptolysin S cluster composed of several components, one of which is SagE. SagE a transmembrane protein with one major extracellular region named ECR. This study aimed to develop a SagE-based DNA candidate vaccine against streptococcosis and examine the immunoprotective mechanism of the vaccine. Results: We constructed a DNA vaccine, pSagE, based on the sagE gene and examined its immunological property in a Japanese flounder (Paralichthys olivaceus) model. The results showed that at 7 days post-vaccination, expression of SagE at transcription and translation levels was detected in the tissues of the vaccinated fish. After challenge with S. iniae at one and two months post-vaccination, pSagE-vaccinated fish exhibited relative percent survival (RPS) of 95% and 88% respectively. Immunological analysis showed that (i) pSagE significantly upregulated the expression of a wide range of immune genes, (ii) pSagE induced the production of specific serum antibodies that bound whole-cell S. iniae, and (iii) treatment of S. iniae with pSagE-induced antibodies blocked bacterial invasion of host cells. To localize the immunoprotective domain of SagE, the ECR-expressing DNA vaccine pSagEECR was constructed. Immunization analysis showed that flounder vaccinated with pSagEECR exhibited a RPS of 68%, and that pSagEECR induced serum antibody production and immune gene expression in a manner similar to, though to lower magnitudes than, those induced by pSagE. Conclusions: We in this study developed a DNA vaccine, pSagE, which induces highly protective immunity against S. iniae. The protective effect of pSagE is probably due to its ability to elicit systemic immune response, in particular that of the humoral branch, which leads to production of specific serum antibodies that impair bacterial infection. These results add insights to the immunoprotective mechanism of fish DNA vaccine.
英文摘要	Background: Streptococcus iniae is a Gram-positive bacterium and a severe pathogen of a wide range of farmed fish. S. iniae possesses a virulence-associated streptolysin S cluster composed of several components, one of which is SagE. SagE a transmembrane protein with one major extracellular region named ECR. This study aimed to develop a SagE-based DNA candidate vaccine against streptococcosis and examine the immunoprotective mechanism of the vaccine.
学科主题	Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Veterinary Sciences
研究领域[WOS]	Veterinary Sciences
关键词[WOS]	FLOUNDER PARALICHTHYS-OLIVACEUS ; TILAPIA OREOCHROMIS-NILOTICUS ; DNA VACCINES ; RAINBOW-TROUT ; VIRULENCE FACTOR ; STREPTOLYSIN-S ; DISEASED FISH ; IDENTIFICATION ; CONSTRUCTION ; VACCINATION
收录类别	SCI
原文出处	10.1186/1751-0147-55-78
语种	英语
WOS记录号	WOS:000328953100001
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16623]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Municipal Hosp, Dept Clin Lab, Qingdao 266000, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yun,Sun, Li,Xing, Ming-qing,et al. SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region[J]. ACTA VETERINARIA SCANDINAVICA,2013,55:78.
APA	Sun, Yun,Sun, Li,Xing, Ming-qing,Liu, Chun-sheng,&Hu, Yong-hua.(2013).SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region.ACTA VETERINARIA SCANDINAVICA,55,78.
MLA	Sun, Yun,et al."SagE induces highly effective protective immunity against Streptococcus iniae mainly through an immunogenic domain in the extracellular region".ACTA VETERINARIA SCANDINAVICA 55(2013):78.