Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta) | |
Wang, Shasha1; Zhao, Fengyang2; Wei, Xiaojiao1; Lu, Bojun1; Duan, Delin3; Wang, Gaoge1 | |
刊名 | JOURNAL OF APPLIED PHYCOLOGY |
2013-08-01 | |
卷号 | 25期号:4页码:1215-1223 |
关键词 | Defense response Elicitor Female gametophyte flg22 Saccharina japonica Phaeophyta |
ISSN号 | 0921-8971 |
通讯作者 | Wang, GG |
中文摘要 | Flg22, which is the most conserved 22-amino acid peptide in the N-terminal part of flagellin, functions as an effective elicitor in higher plants. Marine algae and higher plants share some conserved characteristics on defense response pathways. Flg22-induced defense responses were investigated in female gametophytes of Saccharina japonica. Condensation of chloroplasts and thickened cell walls, as well as relatively stable structures of mitochondria and nucleus indicated that there were hypersensitive programmed cell death occurred after induction by flg22. By using luminol-dependent luminescence detection method, rapid release of H2O2 was detected in the induced female gametophytes and reached a peak concentration of about 46 mu M at 2 h. Reactive oxygen species production was also observed histologically using the fluorescent dye 2',7'-dichlorofluorescein diacetate, showing a consistent result with quantitative analysis of H2O2. Furthermore, results of antioxidant enzyme activities indicated that there was a trend in the order of catalase > superoxide dismutase > glutathione peroxidase. Finally, high level phenol content of cell-free extracts was found after flg22 induction. According to our results, flg22 could be an effective elicitor which could induce defense responses in female gametophytes of S. japonica. |
英文摘要 | Flg22, which is the most conserved 22-amino acid peptide in the N-terminal part of flagellin, functions as an effective elicitor in higher plants. Marine algae and higher plants share some conserved characteristics on defense response pathways. Flg22-induced defense responses were investigated in female gametophytes of Saccharina japonica. Condensation of chloroplasts and thickened cell walls, as well as relatively stable structures of mitochondria and nucleus indicated that there were hypersensitive programmed cell death occurred after induction by flg22. By using luminol-dependent luminescence detection method, rapid release of H2O2 was detected in the induced female gametophytes and reached a peak concentration of about 46 mu M at 2 h. Reactive oxygen species production was also observed histologically using the fluorescent dye 2',7'-dichlorofluorescein diacetate, showing a consistent result with quantitative analysis of H2O2. Furthermore, results of antioxidant enzyme activities indicated that there was a trend in the order of catalase > superoxide dismutase > glutathione peroxidase. Finally, high level phenol content of cell-free extracts was found after flg22 induction. According to our results, flg22 could be an effective elicitor which could induce defense responses in female gametophytes of S. japonica. |
学科主题 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
关键词[WOS] | PROGRAMMED CELL-DEATH ; GRACILARIA-CONFERTA RHODOPHYTA ; BACTERIAL ELICITOR FLAGELLIN ; INNATE IMMUNITY ; OXIDATIVE BURST ; ARABIDOPSIS-THALIANA ; PSEUDOMONAS-AVENAE ; LAMINARIA-DIGITATA ; HYDROGEN-PEROXIDE ; PERCEPTION |
收录类别 | SCI |
原文出处 | 10.1007/s10811-012-9911-4 |
语种 | 英语 |
WOS记录号 | WOS:000321588800032 |
公开日期 | 2014-07-17 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/16616] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Qingdao 39 Middle Sch, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Shasha,Zhao, Fengyang,Wei, Xiaojiao,et al. Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2013,25(4):1215-1223. |
APA | Wang, Shasha,Zhao, Fengyang,Wei, Xiaojiao,Lu, Bojun,Duan, Delin,&Wang, Gaoge.(2013).Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,25(4),1215-1223. |
MLA | Wang, Shasha,et al."Preliminary study on flg22-induced defense responses in female gametophytes of Saccharina japonica (Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 25.4(2013):1215-1223. |
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