Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property
	Chi, Heng; Xiao, Zhi-zhong; Sun, Li
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-09-01
卷号	35 期号:3 页码:972-978
关键词	Nuclear factor 45 Interleukin-2 enhancer binding factor 2 Cynoglossus semilaevis
ISSN号	1050-4648
通讯作者	Sun, L
中文摘要	Nuclear factor 45 (NF45) is a component of the protein complex called nuclear factor of activated T-cells (NFAT), which in mammals regulates interleukin (IL)-2 expression. To date very little is known about fish NF45. In this study, we identified a NF45 (named CsNF45) from half smooth tongue sole Cynoglossus semilaevis and examined its gene organization, expression profile, and regulatory function. We found that CsNF45 is composed of 387 residues and shares 90.3%-97.9% overall sequence identities with the NF45 of human and teleosts. Genetic analysis showed that the genomic sequence of the ORF region of CsNF45 consists of 14 exons and 13 introns. Constitutive expression of CsNF45 occurred in multiple tissues including gill, muscle, brain, heart, liver, head kidney, spleen, and gut. Experimental infection with viral and bacterial pathogens upregulated the expression of CsNF45 in head kidney and spleen in a time-dependent manner. Transient transfection analysis showed that CsNF45 was localized in the nucleus and able to stimulate the activity of mouse IL-2 promoter. These results indicate that CsNF45 possesses immunoregulatory property and is possibly involved in host immune defense against bacterial and viral infection. (C) 2013 Elsevier Ltd. All rights reserved.
英文摘要	Nuclear factor 45 (NF45) is a component of the protein complex called nuclear factor of activated T-cells (NFAT), which in mammals regulates interleukin (IL)-2 expression. To date very little is known about fish NF45. In this study, we identified a NF45 (named CsNF45) from half smooth tongue sole Cynoglossus semilaevis and examined its gene organization, expression profile, and regulatory function. We found that CsNF45 is composed of 387 residues and shares 90.3%-97.9% overall sequence identities with the NF45 of human and teleosts. Genetic analysis showed that the genomic sequence of the ORF region of CsNF45 consists of 14 exons and 13 introns. Constitutive expression of CsNF45 occurred in multiple tissues including gill, muscle, brain, heart, liver, head kidney, spleen, and gut. Experimental infection with viral and bacterial pathogens upregulated the expression of CsNF45 in head kidney and spleen in a time-dependent manner. Transient transfection analysis showed that CsNF45 was localized in the nucleus and able to stimulate the activity of mouse IL-2 promoter. These results indicate that CsNF45 possesses immunoregulatory property and is possibly involved in host immune defense against bacterial and viral infection. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	RNA-BINDING PROTEINS ; ACTIVATED T-CELLS ; HUMAN IL-2 GENE ; TRANSLATIONAL CONTROL ; INTERLEUKIN-2 GENE ; CYCLOSPORINE-A ; IN-VIVO ; RGG BOX ; NF90 ; PROMOTER
收录类别	SCI
原文出处	10.1016/j.fsi.2013.07.016
语种	英语
WOS记录号	WOS:000324511700042
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16611]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chi, Heng,Xiao, Zhi-zhong,Sun, Li. Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):972-978.
APA	Chi, Heng,Xiao, Zhi-zhong,&Sun, Li.(2013).Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property.FISH & SHELLFISH IMMUNOLOGY,35(3),972-978.
MLA	Chi, Heng,et al."Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: Gene structure, expression profile, and immunoregulatory property".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):972-978.