Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China

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	Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China
	Liu, Haizhou 1; Xiao, Lin 1,2; Wei, Jianteng 1; Schmitz, John C.3; Liu, Ming 1; Wang, Cuicui 1; Cheng, Linyou 1; Wu, Ning 1; Chen, Lei1 ; Zhang, Yuyan 4
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2013-07-01
卷号	29 期号:7 页码:1271-1278
关键词	Moderately halophilic actinomycetes Streptomyces 8-O-Methyltetrangulol Naphthomycin A
ISSN号	0959-3993
通讯作者	Lin, XK
中文摘要	A moderately halophilic actinomycetes strain, designated as WH26, was isolated from Weihai Solar Saltern in China. The identification of the strain WH26 was performed by its morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on 16S rRNA sequence comparison. The results showed that the nucleotide sequence of the 16S rRNA gene (1,677 bp) of the strain WH26 exhibited close similarity (97-99 %) with other Streptomyces 16S rRNA genes and the strain WH26 was identified to belong to the genus Streptomyces. An ethyl acetate extraction of Streptomyces sp. nov. WH26 demonstrated significant cellular toxicity. Two compounds, 8-O-methyltetrangulol and naphthomycin A were isolated from the extract via silica gel column chromatography and HPLC. These two compounds showed potent cytotoxic activity against several human tumor cell lines including A549, HeLa, BEL-7402 and HT-29. The present studies suggest that moderately halophilic actinomycetes may be a novel biological source for the discovery of anticancer agents.
英文摘要	A moderately halophilic actinomycetes strain, designated as WH26, was isolated from Weihai Solar Saltern in China. The identification of the strain WH26 was performed by its morphological characteristics, physiological and biochemical tests as well as phylogenetic analysis based on 16S rRNA sequence comparison. The results showed that the nucleotide sequence of the 16S rRNA gene (1,677 bp) of the strain WH26 exhibited close similarity (97-99 %) with other Streptomyces 16S rRNA genes and the strain WH26 was identified to belong to the genus Streptomyces. An ethyl acetate extraction of Streptomyces sp. nov. WH26 demonstrated significant cellular toxicity. Two compounds, 8-O-methyltetrangulol and naphthomycin A were isolated from the extract via silica gel column chromatography and HPLC. These two compounds showed potent cytotoxic activity against several human tumor cell lines including A549, HeLa, BEL-7402 and HT-29. The present studies suggest that moderately halophilic actinomycetes may be a novel biological source for the discovery of anticancer agents.
学科主题	Biotechnology & Applied Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	HALOPHILIC ACTINOMYCETE ; STRUCTURE ELUCIDATION ; BIOLOGICAL-ACTIVITY ; SEQUENCE ALIGNMENT ; NAPHTHOMYCIN ; ANSAMYCIN ; SEDIMENTS ; GENE ; TETRANGOMYCIN ; TETRANGULOL
收录类别	SCI
原文出处	10.1007/s11274-013-1290-8
语种	英语
WOS记录号	WOS:000321976800015
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16591]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao 266109, Shandong, Peoples R China 3.Univ Pittsburgh, Dept Med, Pittsburgh, PA 15213 USA 4.Qingdao Univ Sci & Technol, Sch Chem Engn, Qingdao 266042, Shandong, Peoples R China 5.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
推荐引用方式 GB/T 7714	Liu, Haizhou,Xiao, Lin,Wei, Jianteng,et al. Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(7):1271-1278.
APA	Liu, Haizhou.,Xiao, Lin.,Wei, Jianteng.,Schmitz, John C..,Liu, Ming.,...&Lin, Xiukun.(2013).Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(7),1271-1278.
MLA	Liu, Haizhou,et al."Identification of Streptomyces sp nov WH26 producing cytotoxic compounds isolated from marine solar saltern in China".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.7(2013):1271-1278.