Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment

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	Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment
	Liu, Rui 1; Qiu, Limei 1; Yu, Zuoan 3; Zi, Jun 1; Yue, Feng 1,2; Wang, Lingling 1; Zhang, Huan 1; Teng, Weiming 3; Liu, Xiangfeng 3; Song, Linsheng 1
刊名	JOURNAL OF INVERTEBRATE PATHOLOGY
	2013-10-01
卷号	114 期号:2 页码:144-150
关键词	Pathogencity Bacterial infection Bivalve disease Lesion Low temperature
ISSN号	0022-2011
通讯作者	Song, LS
中文摘要	A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要	A fatal disease with apparent lesions on the adductor muscles of Yesso scallop Patinopecten yessoensis has occurred for three consecutive years from 2009 to 2011 in the northeastern China. This disease has been suspected to be closely associated with bacterial infections. In the present study, 96 bacteria were isolated from the lesions of moribund scallops. They were grouped into Vibrio (88 strains), Moritella (5 strains), Pseudomonas (2 strains) and Shewanella (1 strain) by 16S rDNA sequencing. In the Vibrio group, Vibrio splendidus was the predominant species, consisting of 43 strains. A strain of V. splendidus, named JZ6, was identified as a potential pathogen because it possessed two important virulence factors, Vsm and OmpU. Stain JZ6 was cold-adapted, and could surviveand exhibit haemolytic activity from 4 degrees C to 32 degrees C, with the highest activity observed at 10 degrees C. The pathogenicity of JZ6 was further confirmed by injection and immersion challenges to scallops at 10 degrees C. The cumulative mortality rates in the two, experiments were 80% and 45%, respectively, which were significantly higher (P < 0.05) than those of control and blank groups. The disorganisation of muscle fibres in challenged scallops was observed to be histopathologically identical to that in moribund Yesso scallop collected from the same farm. The results indicate that V. splendidus is the predominantly culturable bacteria from the lesions of diseased Yesso scallop, and JZ6 probably is the pathogenic agent to Yesso scallop in this low temperature environment. (C) 2013 Elsevier Inc. All rights reserved.
学科主题	Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Zoology
研究领域[WOS]	Zoology
关键词[WOS]	BROWN RING DISEASE ; CLAM RUDITAPES-PHILIPPINARUM ; CRASSOSTREA-GIGAS ; PACIFIC OYSTER ; SHELLFISH ; BACTERIA ; TAPETIS ; ALGINOLYTICUS ; MORTALITIES ; RESPONSES
收录类别	SCI
原文出处	10.1016/j.jip.2013.07.005
语种	英语
WOS记录号	WOS:000324790400006
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16586]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Liaoning Ocean & Fisheries Sci Res Inst, Liaoning Key Lab Marine Fisheries Mol Biol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Liu, Rui,Qiu, Limei,Yu, Zuoan,et al. Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2013,114(2):144-150.
APA	Liu, Rui.,Qiu, Limei.,Yu, Zuoan.,Zi, Jun.,Yue, Feng.,...&Song, Linsheng.(2013).Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment.JOURNAL OF INVERTEBRATE PATHOLOGY,114(2),144-150.
MLA	Liu, Rui,et al."Identification and characterisation of pathogenic Vibrio splendidus from Yesso scallop (Patinopecten yessoensis) cultured in a low temperature environment".JOURNAL OF INVERTEBRATE PATHOLOGY 114.2(2013):144-150.