Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei
	Yuan, Jian-Bo 1,2; Zhang, Xiao-Jun 1; Liu, Cheng-Zhang 1; Wei, Jian-Kai1,2 ; Li, Fu-Hua 1; Xiang, Jian-Hai1 ; Zhang, XJ
刊名	BMC EVOLUTIONARY BIOLOGY
	2013-08-06
卷号	13
关键词	Horizontal gene transfer Litopenaeus vannamei Shrimp Bacteria Fungi
ISSN号	1471-2148
通讯作者	Zhang, XJ
中文摘要	Background: In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT). Results: In this study, we analyzed the genome-wide HGT events in L. vannamei. Through homology search and phylogenetic analysis, followed by experimental PCR confirmation, 14 genes with HGT event were identified: 12 of them were transferred from bacteria and two from fungi. Structure analysis of these genes showed that the introns of the two fungi-originated genes were substituted by shrimp DNA fragment, two genes transferred from bacteria had shrimp specific introns inserted in them. Furthermore, around other three bacteria-originated genes, there were three large DNA segments inserted into the shrimp genome. One segment was a transposon that fully transferred, and the other two segments contained only coding regions of bacteria. Functional prediction of these 14 genes showed that 6 of them might be related to energy metabolism, and 4 others related to defense of the organism. Conclusions: HGT events from bacteria or fungi were happened in the genome of L. vannamei, and these horizontally transferred genes can be transcribed in shrimp. This is the first time to report the existence of horizontally transferred genes in shrimp. Importantly, most of these genes are exposed to a negative selection pressure and appeared to be functional.
英文摘要	Background: In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT).
学科主题	Evolutionary Biology ; Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Evolutionary Biology ; Genetics & Heredity
研究领域[WOS]	Evolutionary Biology ; Genetics & Heredity
关键词[WOS]	ESCHERICHIA-COLI ; EXPRESSION ANALYSIS ; DIFFERENTIAL EXPRESSION ; O-METHYLTRANSFERASE ; DNA-REPLICATION ; EVOLUTION ; BACTERIA ; PREDICTION ; TRANSCRIPTOME ; CHROMOSOME
收录类别	SCI
原文出处	10.1186/1471-2148-13-165
语种	英语
WOS记录号	WOS:000323026300001
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16584]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, XJ
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Jian-Bo,Zhang, Xiao-Jun,Liu, Cheng-Zhang,et al. Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei[J]. BMC EVOLUTIONARY BIOLOGY,2013,13.
APA	Yuan, Jian-Bo.,Zhang, Xiao-Jun.,Liu, Cheng-Zhang.,Wei, Jian-Kai.,Li, Fu-Hua.,...&Zhang, XJ.(2013).Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei.BMC EVOLUTIONARY BIOLOGY,13.
MLA	Yuan, Jian-Bo,et al."Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei".BMC EVOLUTIONARY BIOLOGY 13(2013).