Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation
	Chen Huaxin 1; Lin Hanzhi 1,2; Jiang Peng 1; Li Fuchao 1; Qin Song 3
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2013-03-01
卷号	31 期号:2 页码:416-420
关键词	chromatic acclimation electroporation genetic transformation Synechococcus sp CC9311
ISSN号	0254-4059
通讯作者	Jiang, P
中文摘要	Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
英文摘要	Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.
学科主题	Marine & Freshwater Biology ; Oceanography
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Limnology ; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	CHROMATIC ADAPTATION ; MANIPULATION ; DIVERSITY ; MOTILITY ; STRAINS ; GENUS
收录类别	SCI
原文出处	10.1007/s00343-013-2164-5
语种	英语
WOS记录号	WOS:000316018300018
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16579]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Chen Huaxin,Lin Hanzhi,Jiang Peng,et al. Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):416-420.
APA	Chen Huaxin,Lin Hanzhi,Jiang Peng,Li Fuchao,&Qin Song.(2013).Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),416-420.
MLA	Chen Huaxin,et al."Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):416-420.