A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense

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	A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense
	Jiang, Qiufen 1,2; Zhou, Zhi 1; Wang, Leilei 1,2; Wang, Lingling 1; Yue, Feng 1,2; Wang, Jingjing1,2 ; Song, Linsheng 1
刊名	PLOS ONE
	2013-07-26
卷号	8 期号:7 页码:e69158
ISSN号	1932-6203
通讯作者	Wang, LL
中文摘要	Background: Nitric oxide synthase (NOS) is responsible for synthesizing nitric oxide (NO) from L-arginine, and involved in multiple physiological functions. However, its immunological role in mollusc was seldom reported. Methodology: In the present study, an NOS (CfNOS) gene was identified from the scallop Chlamys farreri encoding a polypeptide of 1486 amino acids. Its amino acid sequence shared 50.0 similar to 54.7, 40.7 similar to 47.0 and 42.5 similar to 44.5% similarities with vertebrate neuronal (n), endothelial (e) and inducible (i) NOSs, respectively. CfNOS contained PDZ, oxygenase and reductase domains, which resembled those in nNOS. The CfNOS mRNA transcripts expressed in all embryos and larvae after the 2-cell embryo stage, and were detectable in all tested tissues with the highest level in the gonad, and with the immune tissues hepatopancreas and haemocytes included. Moreover, the immunoreactive area of CfNOS distributed over the haemocyte cytoplasm and cell membrane. After LPS, beta-glucan and PGN stimulation, the expression level of CfNOS mRNA in haemocytes increased significantly at 3 h (4.0-, 4.8- and 2.7-fold, respectively, P < 0.01), and reached the peak at 12 h (15.3- and 27.6-fold for LPS and beta-glucan respectively, P < 0.01) and 24 h (17.3-fold for PGN, P < 0.01). In addition, TNF-alpha also induced the expression of CfNOS, which started to increase at 1 h (5.2-fold, P < 0.05) and peaked at 6 h (19.9-fold, P < 0.01). The catalytic activity of the native CfNOS protein was 30.3 +/- 0.3 U mgprot(-1), and it decreased significantly after the addition of the selective inhibitors of nNOS and iNOS (26.9 +/- 0.4 and 29.3 +/- 0.1 U mgprot(-1), respectively, P < 0.01). Conclusions: These results suggested that CfNOS, with identical structure with nNOS and similar enzymatic characteristics to nNOS and iNOS, played the immunological role of iNOS to be involved in the scallop immune defense against PAMPs and TNF-alpha.
英文摘要	Background: Nitric oxide synthase (NOS) is responsible for synthesizing nitric oxide (NO) from L-arginine, and involved in multiple physiological functions. However, its immunological role in mollusc was seldom reported.
学科主题	Science & Technology - Other Topics
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	NERVOUS-SYSTEM ; MOLECULAR CHARACTERIZATION ; CRASSOSTREA-VIRGINICA ; GECARCINUS-LATERALIS ; EXPRESSION ANALYSIS ; SEQUENCE ALIGNMENT ; MEMORY FORMATION ; CHLAMYS-FARRERI ; POND SNAIL ; LAND CRAB
收录类别	SCI
原文出处	10.1371/journal.pone.0069158
语种	英语
WOS记录号	WOS:000322838900026
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16519]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Qiufen,Zhou, Zhi,Wang, Leilei,et al. A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense[J]. PLOS ONE,2013,8(7):e69158.
APA	Jiang, Qiufen.,Zhou, Zhi.,Wang, Leilei.,Wang, Lingling.,Yue, Feng.,...&Song, Linsheng.(2013).A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense.PLOS ONE,8(7),e69158.
MLA	Jiang, Qiufen,et al."A Scallop Nitric Oxide Synthase (NOS) with Structure Similar to Neuronal NOS and Its Involvement in the Immune Defense".PLOS ONE 8.7(2013):e69158.