A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells
	Wang, Feng-xia 1,2; Wu, Ning 3; Wei, Jian-teng 4,5; Liu, Jia 3; Zhao, Jin 3; Ji, Ai-guo 1; Lin, Xiu-kun 3,6
刊名	BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE
	2013-08-01
卷号	91 期号:4 页码:244-251
关键词	anticancer protein antimetastatic activity Eupolyphaga sinensis beta 1-integrin lung cancer A549 cells
ISSN号	0829-8211
通讯作者	Lin, XK
中文摘要	Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.
英文摘要	Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 mu g/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of beta 1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.
学科主题	Biochemistry & Molecular Biology ; Cell Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	PHASE-II ; CILENGITIDE ; METASTASIS ; INTEGRINS ; ANTIBODY ; GROWTH ; VENOM
收录类别	SCI
原文出处	10.1139/bcb-2013-0002
语种	英语
WOS记录号	WOS:000321891600008
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16516]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Shandong, Peoples R China 2.Shandong Acad Sci, Inst Biol Res, Jinan 250014, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 5.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 6.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China
推荐引用方式 GB/T 7714	Wang, Feng-xia,Wu, Ning,Wei, Jian-teng,et al. A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells[J]. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,2013,91(4):244-251.
APA	Wang, Feng-xia.,Wu, Ning.,Wei, Jian-teng.,Liu, Jia.,Zhao, Jin.,...&Lin, Xiu-kun.(2013).A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells.BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE,91(4),244-251.
MLA	Wang, Feng-xia,et al."A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells".BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 91.4(2013):244-251.