A newly identified anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus with broad spectrum antimicrobial activity

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	A newly identified anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus with broad spectrum antimicrobial activity
	Liu, Yuan 1; Cui, Zhaoxia 1; Li, Xihong 1,2; Song, Chengwen1,2 ; Shi, Guohui1,2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-02-01
卷号	34 期号:2 页码:463-470
关键词	Portunus trituberculatus Anti-lipopolysaccharide factor Expression analysis Antimicrobial activity
ISSN号	1050-4648
通讯作者	Cui, ZX
中文摘要	Anti-lipopolysaccharide factors (ALFs), exhibiting binding and neutralizing activities to lipopolysaccharide (LPS), are the potent antimicrobial peptides of innate immunity in crustaceans. In this study, a unique isoform of ALF (PtALF6) was identified from eyestalk cDNA library of the swimming crab Portunus trituberculatus. The full-length cDNA of PtALF6 was 669 bp encoding 115 amino acids, relatively short to other known ALFs. The deduced peptide of PtALF6 was conserved; it contained the signal peptide and LPS-binding domain, especially the two conserved cysteine residues at both ends of the domain. Predicted tertiary structures of PtALF6 containing four beta-strands and three alpha-helices were similar to that described in Limulus polyphemus. The genomic fragment of PtALF6 contained three exons separated by two introns. Unlike most ALFs expressed in hemocytes, PtALF6 transcript was predominantly detected in gill with 14.05-fold higher than that in hemocytes. After challenge with Vibrio alginolyticus, the temporal expression level of PtALF6 transcript in hemocytes showed a clear time-dependent response expression pattern with two significant peaks at 12 h and 32 h post-injection. The recombinant PtALF6 protein revealed antimicrobial activity against the test Gram-negative and Gram-positive bacteria, but did not inhibit the growth of fungus Pichia pastoris. These results together indicate that PtALF6 is a potential antimicrobial protein against Gram-negative and Gram-positive bacteria infection, and may play an important role in innate immune response of P. trituberculatus. (C) 2012 Elsevier Ltd. All rights reserved.
英文摘要	Anti-lipopolysaccharide factors (ALFs), exhibiting binding and neutralizing activities to lipopolysaccharide (LPS), are the potent antimicrobial peptides of innate immunity in crustaceans. In this study, a unique isoform of ALF (PtALF6) was identified from eyestalk cDNA library of the swimming crab Portunus trituberculatus. The full-length cDNA of PtALF6 was 669 bp encoding 115 amino acids, relatively short to other known ALFs. The deduced peptide of PtALF6 was conserved; it contained the signal peptide and LPS-binding domain, especially the two conserved cysteine residues at both ends of the domain. Predicted tertiary structures of PtALF6 containing four beta-strands and three alpha-helices were similar to that described in Limulus polyphemus. The genomic fragment of PtALF6 contained three exons separated by two introns. Unlike most ALFs expressed in hemocytes, PtALF6 transcript was predominantly detected in gill with 14.05-fold higher than that in hemocytes. After challenge with Vibrio alginolyticus, the temporal expression level of PtALF6 transcript in hemocytes showed a clear time-dependent response expression pattern with two significant peaks at 12 h and 32 h post-injection. The recombinant PtALF6 protein revealed antimicrobial activity against the test Gram-negative and Gram-positive bacteria, but did not inhibit the growth of fungus Pichia pastoris. These results together indicate that PtALF6 is a potential antimicrobial protein against Gram-negative and Gram-positive bacteria infection, and may play an important role in innate immune response of P. trituberculatus. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	BLACK TIGER SHRIMP ; ANTI-LPS FACTOR ; MOLECULAR-CLONING ; ERIOCHEIR-SINENSIS ; PENAEUS-MONODON ; GENOMIC ORGANIZATION ; FACTOR ALF ; ANTIBACTERIAL PEPTIDE ; SCYLLA-PARAMAMOSAIN ; CRYSTAL-STRUCTURE
收录类别	SCI
原文出处	10.1016/j.fsi.2012.11.050
语种	英语
WOS记录号	WOS:000315076100007
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16513]
专题	海洋研究所_海洋生态与环境科学重点实验室海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, EMBL, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuan,Cui, Zhaoxia,Li, Xihong,et al. A newly identified anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus with broad spectrum antimicrobial activity[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):463-470.
APA	Liu, Yuan,Cui, Zhaoxia,Li, Xihong,Song, Chengwen,&Shi, Guohui.(2013).A newly identified anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus with broad spectrum antimicrobial activity.FISH & SHELLFISH IMMUNOLOGY,34(2),463-470.
MLA	Liu, Yuan,et al."A newly identified anti-lipopolysaccharide factor from the swimming crab Portunus trituberculatus with broad spectrum antimicrobial activity".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):463-470.