Effects of cultivation years on effective constituent content of Fritillaria pallidiflora Schernk

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	Effects of cultivation years on effective constituent content of Fritillaria pallidiflora Schernk
	Tan, Y.1,2,3 ; Wang, Q. -Q.1; Gao, T. -T.1; Ma, Y.1; Shao, H. -B.2; Shao, C. -Y.4
刊名	PLANT BIOSYSTEMS
	2013-12-01
卷号	147 期号:4 页码:1184-1190
关键词	Fritillaria pallidiflora Schernk cultivation years growth and development imperialine polysaccharide HPLC
ISSN号	1126-3504
通讯作者	Shao, HB (reprint author), Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China. shaohongbochu@126.com ; shaochuyang34@126.com
产权排序	[Tan, Y.; Wang, Q. -Q.; Gao, T. -T.; Ma, Y.] Shihezi Univ, Coll Pharm, Shihezi 832002, Xinjiang, Peoples R China; [Tan, Y.; Shao, H. -B.] Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China; [Tan, Y.] Minist Educ, Key Lab Xinjiang Phytomed Resources, Shihezi 832002, Xinjiang, Peoples R China; [Shao, C. -Y.] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
中文摘要	Fritillaria pallidiflora Schrenk has been treasured in traditional classic medicine as an antitussive, antiasthmatic and expectorant for hundreds of years. With gradually decreasing wild F. pallidiflora resources, the herb can no longer satisfy the demand. Artificial cultivation is one of the most effective ways to solve the contradiction between supply and demand in the medicinal material market. During the growth of Rhizomes medicinal plants, root biomass and active ingredient content showed dynamic accumulated variation with increasing cultivation years. Up to now, hardly any attempts have been made to investigate the relationship between quality and cultivation years of F. pallidiflora. Therefore, in this paper, we determined the optimum harvesting time by comparing biomass and biological characteristics of F. pallidiflora at different cultivation times. High-performance liquid chromatography with evaporative light scattering detection and phenol-sulfuric acid visible spectrophotometry was performed to determine imperialine and polysaccharide content of F. pallidiflora bulbs. From year 1 to 6 of cultivation, we observed an upward trend in plant height, diameter and dry weight of F. pallidiflora, while water content decreased. Plant height and dry weight increased remarkably during the fourth year of cultivation. The content of imperialine and polysaccharide of F. pallidiflora bulbs, on the other hand, showed an upward trend from year 1 to 3, after which it decreased from year 3 to 6. By comparing plant growth, biomass development and the accumulation of imperialine and polysaccharide, the best harvesting time of F. pallidiflora was determined to be after 4 years of cultivation. Our results showed that it is possible to establish a safe, effective, stable and controllable production process, which could play an important role in achieving sustainable utilization of F. pallidiflora resources.
英文摘要	Fritillaria pallidiflora Schrenk has been treasured in traditional classic medicine as an antitussive, antiasthmatic and expectorant for hundreds of years. With gradually decreasing wild F. pallidiflora resources, the herb can no longer satisfy the demand. Artificial cultivation is one of the most effective ways to solve the contradiction between supply and demand in the medicinal material market. During the growth of Rhizomes medicinal plants, root biomass and active ingredient content showed dynamic accumulated variation with increasing cultivation years. Up to now, hardly any attempts have been made to investigate the relationship between quality and cultivation years of F. pallidiflora. Therefore, in this paper, we determined the optimum harvesting time by comparing biomass and biological characteristics of F. pallidiflora at different cultivation times. High-performance liquid chromatography with evaporative light scattering detection and phenol-sulfuric acid visible spectrophotometry was performed to determine imperialine and polysaccharide content of F. pallidiflora bulbs. From year 1 to 6 of cultivation, we observed an upward trend in plant height, diameter and dry weight of F. pallidiflora, while water content decreased. Plant height and dry weight increased remarkably during the fourth year of cultivation. The content of imperialine and polysaccharide of F. pallidiflora bulbs, on the other hand, showed an upward trend from year 1 to 3, after which it decreased from year 3 to 6. By comparing plant growth, biomass development and the accumulation of imperialine and polysaccharide, the best harvesting time of F. pallidiflora was determined to be after 4 years of cultivation. Our results showed that it is possible to establish a safe, effective, stable and controllable production process, which could play an important role in achieving sustainable utilization of F. pallidiflora resources.
学科主题	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	BIODIVERSITY ; PLANTS ; FUNGI ; WILD ; L.
收录类别	SCI
资助信息	National Natural Science Fund [30860022]; Natural Science Fund Key Program of Shihezi University [ZRKX2009ZD05]; CAS/SAFEA International Partnership Program for Creative Research Teams
原文出处	http://dx.doi.org/10.1080/11263504.2013.859181
语种	英语
WOS记录号	WOS:000328536300034
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/7035]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Shihezi Univ, Coll Pharm, Shihezi 832002, Xinjiang, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Biol & Bioresources Utilizat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China 3.Minist Educ, Key Lab Xinjiang Phytomed Resources, Shihezi 832002, Xinjiang, Peoples R China 4.Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Tan, Y.,Wang, Q. -Q.,Gao, T. -T.,et al. Effects of cultivation years on effective constituent content of Fritillaria pallidiflora Schernk[J]. PLANT BIOSYSTEMS,2013,147(4):1184-1190.
APA	Tan, Y.,Wang, Q. -Q.,Gao, T. -T.,Ma, Y.,Shao, H. -B.,&Shao, C. -Y..(2013).Effects of cultivation years on effective constituent content of Fritillaria pallidiflora Schernk.PLANT BIOSYSTEMS,147(4),1184-1190.
MLA	Tan, Y.,et al."Effects of cultivation years on effective constituent content of Fritillaria pallidiflora Schernk".PLANT BIOSYSTEMS 147.4(2013):1184-1190.