A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane
	Liu, Fanghua
刊名	ENERGY & ENVIRONMENTAL SCIENCE
	2014-01
卷号	7 期号:1 页码:408-415
关键词	RICE FIELD SOIL GEOBACTER-SULFURREDUCENS ACETATE RNA MICROORGANISMS BACTERIA HYDROGEN FORMATE IDENTIFICATION ASSOCIATION
ISSN号	1754-5692
通讯作者	Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. arotaru@microbio.umass.edu
产权排序	[Rotaru, Amelia-Elena; Shrestha, Pravin Malta; Liu, Fanghua; Shrestha, Minita; Shrestha, Devesh; Wardman, Colin; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Liu, Fanghua] Yantai Inst Coastal Res, Yantai, Peoples R China; [Embree, Mallory; Zengler, Karsten] Univ Calif San Diego, La Jolla, CA 92093 USA
中文摘要	Anaerobic conversion of organic wastes and biomass to methane is an important bioenergy strategy, which depends on poorly understood mechanisms of interspecies electron transfer to methanogenic microorganisms. Metatranscriptomic analysis of methanogenic aggregates from a brewery wastewater digester, coupled with fluorescence in situ hybridization with specific 16S rRNA probes, revealed that Methanosaeta species were the most abundant and metabolically active methanogens. Methanogens known to reduce carbon dioxide with H2 or formate as the electron donor were rare. Although Methanosaeta have previously been thought to be restricted to acetate as a substrate for methane production, Methanosaeta in the aggregates had a complete complement of genes for the enzymes necessary for the reduction of carbon to methane, and transcript abundance for these genes was high. Furthermore, Geobacter species, the most abundant bacteria in the aggregates, highly expressed genes for ethanol metabolism and for extracellular electron transfer via electrically conductive pili, suggesting that Geobacter and Methanosaeta species were exchanging electrons via direct interspecies electron transfer (DIET). This possibility was further investigated in defined co-cultures of Geobacter metallireducens and Methanosaeta harundinacea which stoichiometrically converted ethanol to methane. Transcriptomic, radiotracer, and genetic analysis demonstrated that M. harundinacea accepted electrons via DIET for the reduction of carbon dioxide to methane. The discovery that Methanosaeta species, which are abundant in a wide diversity of methanogenic environments, are capable of DIET has important implications not only for the functioning of anaerobic digesters, but also for global methane production.
学科主题	Chemistry, Multidisciplinary ; Energy & Fuels ; Engineering, Chemical ; Environmental Sciences
收录类别	SCI
资助信息	Office of Science (BER), U.S. Department of Energy [DE-SC0004485]
原文出处	http://dx.doi.org/10.1039/c3ee42189a
语种	英语
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/7026]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
推荐引用方式 GB/T 7714	Liu, Fanghua. A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J]. ENERGY & ENVIRONMENTAL SCIENCE,2014,7(1):408-415.
APA	Liu, Fanghua.(2014).A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane.ENERGY & ENVIRONMENTAL SCIENCE,7(1),408-415.
MLA	Liu, Fanghua."A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane".ENERGY & ENVIRONMENTAL SCIENCE 7.1(2014):408-415.