Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis

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	Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis
	Cui, Hongli 1,2; Yu, Xiaona 3; Wang, Yan1 ; Cui, Yulin1 ; Li, Xueqin 4; Liu, Zhaopu 3; Qin, Song1
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2014-02-01
卷号	26 期号:1 页码:91-103
关键词	Xanthophylls biosynthesis Carotenoid hydroxylase Haematococcus pluvialis Gene cloning High light
ISSN号	0921-8971
通讯作者	Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. sea@njau.edu.cn ; sqin@yic.ac.cn
产权排序	[Cui, Hongli; Wang, Yan; Cui, Yulin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Cui, Hongli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Xiaona; Liu, Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Li, Xueqin] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
中文摘要	A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.
英文摘要	A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	CHLORELLA-ZOFINGIENSIS CHLOROPHYTA ; ASTAXANTHIN BIOSYNTHETIC-PATHWAY ; FUNCTIONAL-ANALYSIS ; BETA-RING ; CLUSTAL-W ; ARABIDOPSIS ; ACCUMULATION ; LUTEIN ; PLANTS ; LIGHT
收录类别	SCI
资助信息	National Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong province comprehensive strategic cooperation project of the Chinese Academy of Sciences [2011A090100040]; Science and Technology Planning Project of Yantai, China [2010247]
原文出处	http://dx.doi.org/10.1007/s10811-013-0055-y
语种	英语
WOS记录号	WOS:000330969800012
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/7016]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China 4.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China
推荐引用方式 GB/T 7714	Cui, Hongli,Yu, Xiaona,Wang, Yan,et al. Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(1):91-103.
APA	Cui, Hongli.,Yu, Xiaona.,Wang, Yan.,Cui, Yulin.,Li, Xueqin.,...&Qin, Song.(2014).Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis.JOURNAL OF APPLIED PHYCOLOGY,26(1),91-103.
MLA	Cui, Hongli,et al."Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis".JOURNAL OF APPLIED PHYCOLOGY 26.1(2014):91-103.