Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress

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	Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress
	Liu Zhixiang 1,2; Cui Hongli 2; Liu Zhengyi 1,2; Wang Yinchu2 ; Cui Yulin2 ; Liu Zhaopu 1; Qin Song2
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2014-03-01
卷号	32 期号:2 页码:403-418
关键词	Synechocystis sp PCC6803 cyanobacteria cDNA microarray transcriptomics low temperature stress
ISSN号	0254-4059
通讯作者	Liu, ZP (reprint author), Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China. sea@njau.edu.cn ; sqin@yic.ac.cn
产权排序	[Liu Zhixiang; Liu Zhengyi; Liu Zhaopu] Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China; [Liu Zhixiang; Cui Hongli; Liu Zhengyi; Wang Yinchu; Cui Yulin; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
中文摘要	In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.
英文摘要	In this study, cDNA microarrays were developed from 3569 mRNA reads to analyze the expression profiles of the transcriptomes of Synechocystis sp. PCC6803 under low temperature (LT) stress. Among the genes on the cDNA microarrays, 899 LT-affected genes exhibited a 1.5-fold (or greater) difference in expression compared with the genes from normal unstressed Synechocystis sp. PCC6803. Of the differentially expressed genes, 353 were up-regulated and 246 were down-regulated. The results showed that genes involved in photosynthesis were activated at LT (10A degrees C), including genes for photosystem I, photosystem II, photosynthetic electron transport, and cytochrome b6/f complex. Moreover, desB, one of four genes that encode the fatty acid desaturases, was also induced by LT. However, the LT conditions to some degree enhanced the transcription of some genes. In addition, LT (10A degrees C) may reduce cellular motility by regulating the transcription of spkA (sll1575), a serine/threonine protein kinase. The results reported in this study may contribute to a better understanding of the responses of the Synechocystis cell to LT, including pathways involved in photosynthesis and repair.
学科主题	Limnology ; Oceanography
研究领域[WOS]	Marine & Freshwater Biology ; Oceanography
关键词[WOS]	COLD-REGULATED GENES ; SP STRAIN PCC-6803 ; PROTEIN-KINASE ; CYANOBACTERIUM SYNECHOCYSTIS ; SIGNAL-TRANSDUCTION ; UNICELLULAR CYANOBACTERIUM ; MEMBRANE-FLUIDITY ; ARABIDOPSIS-THALIANA ; STRUCTURAL-ANALYSIS ; OMEGA-3 DESATURASE
收录类别	SCI
资助信息	National Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Outstanding Young Scholars Fellowship of Shandong Province [JQ200914]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong Province Comprehensive Strategic Cooperation Project of the Chinese Academy of Sciences [2011A090100040]
原文出处	http://dx.doi.org/10.1007/s00343-014-3174-7
语种	英语
WOS记录号	WOS:000332739600018
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/7010]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Agr Univ, Key Lab Marine Biol, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Liu Zhixiang,Cui Hongli,Liu Zhengyi,et al. Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2014,32(2):403-418.
APA	Liu Zhixiang.,Cui Hongli.,Liu Zhengyi.,Wang Yinchu.,Cui Yulin.,...&Qin Song.(2014).Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,32(2),403-418.
MLA	Liu Zhixiang,et al."Transcriptomic analysis of Synechocystis sp PCC6803 under low-temperature stress".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 32.2(2014):403-418.