纤维质原料微生物转化与生物量全利用的研究

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题名	纤维质原料微生物转化与生物量全利用的研究
作者	陈洪章
学位类别	博士
答辩日期	1998-05-01
授予单位	中国科学院研究生院
导师	李佐虎
中文摘要	纤维质原料生物量全利用为研究目标，实物多学科，多技术的结合，形成技术经济上的新突破口。本文研究了麦草汽爆分离技术路线，通过红外光谱，电子能谱，电镜等方法对汽爆的作用机制作了初步分析。汽爆能使80%纤维素自体水解成水溶性，还可使部分木质素裂解，再用乙醇萃取法使75%木质素进一步与纤维素分离。采用周期变压变温固态发酵新技术，以汽爆麦草作底物，可使纤维素酶生产成本可大幅度降低。根据外界周期激新思路，通过补料与周期变温控制克服了糖化与发酵在温度上的矛盾。首次应用汽爆麦草作原料进行了木质素酶固态发酵方法的初步探索。以汽爆麦草为基质，不加黎芦醇，也能诱导木质素酶生成，LiP和MnP生产率分别达到了21.667U/L.h和11.458U/L.h。提出了纤维质原料生物量全利用的新思路；探讨了“生态工业”的概念设计及其发展模式。
索取号	纤维质原料 ; 生物量全利用 ; 组分分离 ; 微生物转化 ; 生态工业
英文摘要	For the request of large scale industrial design, the integration of technique and economic study on the new idea, new process and equipment are developed to break through the bottle neck of the economic and technique. The separation of tree component is essential. With analysis by IR, XPS, SEM, the study on the process of steam exploded separation was carry out to approve that 80% hemicellulose is self-hydrolysis and become water-solubility and then 75% lignin is decomposed and extracted by alcohol. With the new technique of pressure and temperature oscillation solid fermentation, the filter paper activity of cellulase is increased by about ten times and βGase activity approach to the level of FPA used the steam exploded wheat straw as substrate. The lower investment and production cost could be obtained by the technique. On the basis of the principle of period oscillation, it is overcome the temperature inconsistence between saccharification and fermentation using fed-batch and temperature oscillation. The result is the ethanol concentration of 5.4% and the yield of 0.22. Now the lignin enzyme production exists some problems that the fermentation period is long and the production cost is high, which use complex media and must add 3,4-dimethoxybenzyl alcohol. The solid state fermentation of lignin enzyme was investigated. Used the steam exploded wheat straw as substrate and without adding of 3,4-dimethoxybenzyl alcohol, the rates of production in LiP and MnP are 21.667U/L.h and 11.458U/L.h respectively. New ideas of lignocellulose biomass utilization were brought forward from the view point of green chemistry. The concept design and development models of ecological industry were investigated. We are facing with the researchful goal and task in lignocellulose utilization from the strategic altitude.
语种	中文
公开日期	2014-06-25
页码	171
内容类型	学位论文
源URL	[http://ir.ipe.ac.cn/handle/122111/8325]
专题	过程工程研究所_研究所（批量导入）
推荐引用方式 GB/T 7714	陈洪章. 纤维质原料微生物转化与生物量全利用的研究[D]. 中国科学院研究生院. 1998.