Effects of polycyclic musks HHCB and AHTN on steroidogenesis in H295R cells

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Effects of polycyclic musks HHCB and AHTN on steroidogenesis in H295R cells
	Li, Zhuona ; Yin, Nuoya ; Liu, Qian ; Wang, Chang ; Wang, Thanh ; Wang, Yichen ; Qu, Guangbo ; Liu, Jiyan ; Cai, Yaqi ; Zhou, Qunfang ; Jiang, Guibin
刊名	CHEMOSPHERE
	2013
卷号	90 期号:3 页码:1227-1235
通讯作者	Zhou, QF
中文摘要	1,3,4,6,7,8-Hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-(gamma)-2-benzopyran (HHCB) and 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphthalene (AHTN) are widely used in personal care products. Previous studies showed that HHCB and AHTN can be found in various environmental matrices and have potential endocrine disrupting effects. However, the effects on adrenocortical function of HHCB and AHTN are not fully understood. This study evaluated the influences of HHCB and AHTN on seven steroid hormones (progesterone, aldosterone, cortisol, 17 alpha-OH-progesterone, androstenedione, 17 beta-estradiol, and testosterone) and 10 genes involved in steroidogenic pathways (HMGR, StAR, CYP11A1, 3 beta HSD2, CYP17, CYP21, CYP11B1, CYP11B2, 17 beta HSD, and CYP19) using the H295R cell line in the absence and presence of 8-Br-cAMP. MC2R transcription on the cell membrane was also examined to further investigate the effects of HHCB and AHTN on adrenal steroidogenesis. The results demonstrated that HHCB and AHTN could inhibit progesterone and cortisol production mainly by the suppression of 3 beta HSD2 and CYP21. Meanwhile, high concentrations of AHTN can affect the sensitivity of H295R cells to ACTH by disrupting MR transcription. Overall, the results indicate that high concentrations of HHCB and AHTN can affect steroidogenesis in vitro using the H295R cell line. (C) 2012 Elsevier Ltd. All rights reserved.
收录类别	SCI
WOS记录号	WOS:000312978700044
公开日期	2014-06-06
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7553]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Li, Zhuona,Yin, Nuoya,Liu, Qian,et al. Effects of polycyclic musks HHCB and AHTN on steroidogenesis in H295R cells[J]. CHEMOSPHERE,2013,90(3):1227-1235.
APA	Li, Zhuona.,Yin, Nuoya.,Liu, Qian.,Wang, Chang.,Wang, Thanh.,...&Jiang, Guibin.(2013).Effects of polycyclic musks HHCB and AHTN on steroidogenesis in H295R cells.CHEMOSPHERE,90(3),1227-1235.
MLA	Li, Zhuona,et al."Effects of polycyclic musks HHCB and AHTN on steroidogenesis in H295R cells".CHEMOSPHERE 90.3(2013):1227-1235.