The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells
	Cheng, Lin 1; Xia, Tian-Song 1; Wang, Yi-Fen2 ; Zhou, Wenbin 1; Liang, Xiu-Qing 1; Xue, Jin-Qiu 1; Shi, Liang 1; Wang, Ying 1; Ding, Qiang 1
刊名	PLOS ONE
	2014-03-06
卷号	9 期号:3 页码:e90848
关键词	OXYGEN SPECIES GENERATION BCL-2 FAMILY PROTEINS PLATYCODON-GRANDIFLORUM GINSENOSIDE RH2 HEPATOMA-CELLS MAPK PATHWAYS ANTICANCER ACTIVATION INDUCTION DEATH
ISSN号	1932-6203
通讯作者	Ding, Q (reprint author), Nanjing Med Univ, Affiliated Hosp 1, Jiangsu Breast Dis Ctr, Nanjing, Jiangsu, Peoples R China. ; dingqiang@njmu.edu.cn
英文摘要	There is growing interest in development of natural products as anti-cancer and chemopreventive agents. Many triterpenoids have been proved as potential agents for chemoprevention and therapy of breast cancer. Ginsenosides from ginseng, which mostly belong to dammarane-type triterpenoids, have gained great attention for their anti-breast cancer activity with diverse mechanisms. However, studies of other kinds of triterpenoid saponins on breast cancer are limited. Previously, we purified and identified a novel oleanane-type triterpene saponin named D Rhamnose beta-hederin (DR beta-H) from Clematis ganpiniana, a Chinese traditional anti-tumor herb. In the present study, DR beta-H showed strong inhibitory activity on the growth of various breast cancer cells and induced apoptosis in these cells. DR beta-H inhibited PI3K/AKT and activated ERK signaling pathway. PI3K inhibitor LY294002 synergistically enhanced DR beta-H-induced apoptosis whereas MEK inhibitor U0126 reduced the apoptosis rate. Moreover, DR beta-H regulated the ratio of pro-apoptotic and anti-apoptotic Bcl-2 family proteins. Furthermore, DR beta-H induced depolarization of mitochondrial membrane potential which released Apaf-1 and Cytochrome C from the inter membrane space into the cytosol, where they promoted caspase-9 and caspase-3 activation. This is the first report on the pro-apoptotic effects of DR beta-H, a novel oleanane-type triterpenoid saponin, on breast cancer cells and its comprehensive apoptosis pathways. It implied that oleanane-type triterpenoid saponin DR beta-H could be a promising candidate for chemotherapy of breast cancer.
学科主题	Multidisciplinary Sciences
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	OXYGEN SPECIES GENERATION ; BCL-2 FAMILY PROTEINS ; PLATYCODON-GRANDIFLORUM ; GINSENOSIDE RH2 ; HEPATOMA-CELLS ; MAPK PATHWAYS ; ANTICANCER ; ACTIVATION ; INDUCTION ; DEATH
收录类别	SCI
资助信息	Natural Science Foundation of China [81272916, 81202077]; Natural Science Foundation of Jiangsu Province [BK2011855]; key projects of Jiangsu Provincial Health Office [H201110]; Project of Jiangsu Province Traditional Chinese medicine bureau [LZ11084]; Six Talents Peak projects of Jiangsu province; Priority Academic Program Development of Jiangsu higher Education Institutions (PAPD)
语种	英语
WOS记录号	WOS:000332483600095
公开日期	2014-05-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18051]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Nanjing Med Univ, Affiliated Hosp 1, Jiangsu Breast Dis Ctr, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Lin,Xia, Tian-Song,Wang, Yi-Fen,et al. The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells[J]. PLOS ONE,2014,9(3):e90848.
APA	Cheng, Lin.,Xia, Tian-Song.,Wang, Yi-Fen.,Zhou, Wenbin.,Liang, Xiu-Qing.,...&Ding, Qiang.(2014).The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells.PLOS ONE,9(3),e90848.
MLA	Cheng, Lin,et al."The Apoptotic Effect of D Rhamnose beta-Hederin, a Novel Oleanane-Type Triterpenoid Saponin on Breast Cancer Cells".PLOS ONE 9.3(2014):e90848.