Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus.

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 植物代谢工程团队

	Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus.
	Ma, Y. B. ; Zhang, Z. F. ; Shao, M. Y. ; Kang, K. H. ; Shi, X. L. ; Dong, Y. P. ; Li, J. L.
刊名	Mar Biotechnol (NY)
	2012
卷号	14 期号:2 页码:245-251
关键词	Mitochondria Sulfide detoxification Sulfide:quinone oxidoreductase (SQR) Urechis unicinctus
中文摘要	Sulfide is a natural, widely distributed, poisonous substance, and sulfide:quinone oxidoreductase (SQR) is responsible for the initial oxidation of sulfide in mitochondria. In this study, we examined the response of SQR to sulfide exposure (25, 50, and 150 μM) at mRNA, protein, and enzyme activity levels in the body wall and hindgut of the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. The results revealed SQR mRNA expression during sulfide exposure in the body wall and hindgut increased in a time- and concentrationdependent manner that increased significantly at 12 h and continuously increased with time. At the protein level, SQR expression in the two tissues showed a time-dependent relationship that increased significantly at 12 h in 50 μM sulfide and 6 h in 150 μM, and then continued to increase with time while no significant increase appeared after 25 μM sulfide exposure. SQR enzyme activity in both tissues increased significantly in a time-dependent manner after 50 μM sulfide exposure. We concluded that SQR expression could be induced by sulfide exposure and that the two tissues studied have dissimilar sulfide metabolic patterns. A U. unicinctus sulfide-induced detoxification mechanism was also discussed.
学科主题	能源植物资源
收录类别	SCI
语种	英语
公开日期	2014-03-27
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1741]
专题	青岛生物能源与过程研究所_植物代谢工程团队
推荐引用方式 GB/T 7714	Ma, Y. B.,Zhang, Z. F.,Shao, M. Y.,et al. Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus.[J]. Mar Biotechnol (NY),2012,14(2):245-251.
APA	Ma, Y. B..,Zhang, Z. F..,Shao, M. Y..,Kang, K. H..,Shi, X. L..,...&Li, J. L..(2012).Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus..Mar Biotechnol (NY),14(2),245-251.
MLA	Ma, Y. B.,et al."Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus.".Mar Biotechnol (NY) 14.2(2012):245-251.