Microarray-based fluorescence assay of endonuclease functionality and inhibition

	Microarray-based fluorescence assay of endonuclease functionality and inhibition
	Ma L ; Su M ; Li T ; Wang ZX
刊名	analyst
	2013
卷号	138 期号:4 页码:1048-1052
关键词	LINKED-IMMUNOSORBENT-ASSAY RESTRICTION-ENDONUCLEASE DNA-REPLICATION SENSITIVE DETECTION CIRCULAR-DICHROISM GRAPHENE OXIDE ENZYME CLEAVAGE RECOMBINATION BINDING
ISSN号	0003-2654
通讯作者	wang zx
中文摘要	here, a double-strand (ds) dna microarray-based fluorescence assay has been deployed for studying endonuclease functionality and inhibition. the dsdna microarrays are fabricated by hybridization of the cy5-labeled oligonucleotides with the immobilized complementary oligonucleotide probes on the glass slide. the microarray displays significant fluorescence decrease in response to endonuclease, which is able to cleave the oligonucleotide moiety of dsdna. two endonucleases, ecori and bamhi, and four potential inhibitors, doxorubicin hydrochloride (dox), 5-fluorouracil (5-fu), ethidium bromide (eb) and actinomycin d (actd), were selected to address the feasibility of this assay. enzyme activities of the endonucleases are detected with high specificity down to the limits of 1.1 u ml(-1) for ecori and 2.0 u ml(-1) for bamhi, respectively. in addition, bamhi and ecori inhibition by the inhibitors are also shown, demonstrating the potential for high-throughput screening for inhibitors.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000313805200013
公开日期	2014-04-18
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50393]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Ma L,Su M,Li T,et al. Microarray-based fluorescence assay of endonuclease functionality and inhibition[J]. analyst,2013,138(4):1048-1052.
APA	Ma L,Su M,Li T,&Wang ZX.(2013).Microarray-based fluorescence assay of endonuclease functionality and inhibition.analyst,138(4),1048-1052.
MLA	Ma L,et al."Microarray-based fluorescence assay of endonuclease functionality and inhibition".analyst 138.4(2013):1048-1052.