Luteinizing-hormone-releasing-hormone-containing biodegradable polymer micelles for enhanced intracellular drug delivery

	Luteinizing-hormone-releasing-hormone-containing biodegradable polymer micelles for enhanced intracellular drug delivery
	Wang R ; Hu XL ; Yue J ; Zhang WJ ; Cai LY ; Xie ZG ; Huang YB ; Jing XB
刊名	journal of materials chemistry b
	2013
卷号	1 期号:3 页码:293-301
关键词	NANOPARTICLES CONJUGATE CHEMOTHERAPY DOXORUBICIN RECEPTORS SYSTEMS DESIGN
ISSN号	2050-750x
通讯作者	wang r
中文摘要	a biodegradable triblock copolymer, poly(ethylene oxide)-block-poly(allyl glycidyl ether)-block-poly-(dl-lactide) (mpeg-b-page-b-pla), with allyl groups on its middle block was designed and synthesized through anionic polymerization of allyl glycidyl ether (age) with peg monomethyl ether sodium salt as the macroinitiator and subsequent ring-opening polymerization of dl-lactide. luteinizing hormone-releasing hormone (lhrh) was conjugated to the page block through a linkage of -sch2ch2c(=o)nh-between the allyl and lhrh residues. the lhrh content in the conjugate was ca. 25 wt%. owing to the amphiphilic nature of the conjugate, it was self-assembled into micelles of 15-40 nm in diameter and with the lhrh moieties in the hydrophilic corona of the micelles. cellular uptake experiments were carried out using flow cytometry and confocal laser scanning microscopy (clsm) with hela cells as lhrh-receptor overexpressing cells and hepg-2 cells as normal cells. hela cells displayed more cellular uptake of the lhrh-micelles than the normal cells. in vivo biodistribution of the lhrh-containing micelles and lhrh-free micelles was studied using a cri maestro (tm) imaging system. preferred accumulation in tumor sites of lhrh-containing micelles was observed at 24 hours post injection. therefore, lhrh-conjugated amphiphilic copolymers might be used as a potential drug delivery system for the treatment of lhrh receptor overexpressing carcinoma.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000314787400005
公开日期	2014-04-18
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50358]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang R,Hu XL,Yue J,et al. Luteinizing-hormone-releasing-hormone-containing biodegradable polymer micelles for enhanced intracellular drug delivery[J]. journal of materials chemistry b,2013,1(3):293-301.
APA	Wang R.,Hu XL.,Yue J.,Zhang WJ.,Cai LY.,...&Jing XB.(2013).Luteinizing-hormone-releasing-hormone-containing biodegradable polymer micelles for enhanced intracellular drug delivery.journal of materials chemistry b,1(3),293-301.
MLA	Wang R,et al."Luteinizing-hormone-releasing-hormone-containing biodegradable polymer micelles for enhanced intracellular drug delivery".journal of materials chemistry b 1.3(2013):293-301.