pH-Triggered Charge-Reversal Polypeptide Nanoparticles for Cisplatin Delivery: Preparation and In Vitro Evaluation

	pH-Triggered Charge-Reversal Polypeptide Nanoparticles for Cisplatin Delivery: Preparation and In Vitro Evaluation
	Huang Y ; Tang ZH ; Zhang XF ; Yu HY ; Sun H ; Pang X ; Chen XS
刊名	biomacromolecules
	2013
卷号	14 期号:6 页码:2023-2032
关键词	NUCLEAR DRUG-DELIVERY COPOLYMERS MICELLE CELLS ACID) INTERNALIZATION NANOTECHNOLOGY NANOMEDICINE NANOCARRIERS DOXORUBICIN
ISSN号	1525-7797
通讯作者	tang zh
中文摘要	a series of ph-responsive random copolymer poly(l-glutamic acid-co-l-lysine) [p(glu-co-lys)] were synthesized through the ring-opening polymerization (rop) of gamma-benzyl-t-glutamate n-carboxyanhydride (blg-nca) and 3-benzyloxycarbonyl-l-lysine n-carboxyanhydride (zlys-nca) and the subsequent deprotection. the chemical structure of the p(glu-co-lys)s was confirmed by nmr. critical aggregation concentration and transmission electron microscopy measurements indicated that the p(glu-co-lys)s could self-assemble into aggregates in phosphate buffer. the surface charge of p(glu-co-lys) aggregates was greatly affected by the solution's ph and l-glutamic acid/l-lysine ratio because the carboxyl and amino groups present on the p(glu-co-lys) aggregates could be protonated or deprotonated to become charged. the ph value of the solution at which the surface charge of the p(glu-co-lys) aggregates reversed could be manipulated by the feed ratio of blg-nca and zlys-nca. in vitro methyl thiazolyl tetrazolium assays demonstrated that negatively charged p(glu-co-lys)s were nontoxic and biocompatible. positive charged p(glu-co-lys)s showed some cytotoxicity to he la cells. cisplatin (cddp) was used as a model anticancer drug to evaluate the charge-reversal drug delivery system. by the manipulation of cddp loading content, the surface charge of the cddp/p(glu-co-lys) nanoparticles could be reversed to positive from negative at tumor extracellular ph (ph, 6.5-7.2). an enhanced drug uptake and inhibition of cancer cell proliferation were observed for the tumoral ph(e) triggered charge-reversal cddp/p(glu-co-lys) drug delivery system. these indicated that the cddp/p(glu-colys) nanoparticles could be used as intelligent drug delivery systems for cancer therapy.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000320356000036
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49830]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Huang Y,Tang ZH,Zhang XF,et al. pH-Triggered Charge-Reversal Polypeptide Nanoparticles for Cisplatin Delivery: Preparation and In Vitro Evaluation[J]. biomacromolecules,2013,14(6):2023-2032.
APA	Huang Y.,Tang ZH.,Zhang XF.,Yu HY.,Sun H.,...&Chen XS.(2013).pH-Triggered Charge-Reversal Polypeptide Nanoparticles for Cisplatin Delivery: Preparation and In Vitro Evaluation.biomacromolecules,14(6),2023-2032.
MLA	Huang Y,et al."pH-Triggered Charge-Reversal Polypeptide Nanoparticles for Cisplatin Delivery: Preparation and In Vitro Evaluation".biomacromolecules 14.6(2013):2023-2032.