Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA

	Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA
	Dong X ; Lin L ; Chen J ; Tian H ; Xiao C ; Guo Z ; Li Y ; Wei Y ; Chen X
刊名	acta biomaterialia
	2013
卷号	9 期号:6 页码:6943-6952
关键词	GENE DELIVERY SIRNA DELIVERY DNA DELIVERY LINKED OLIGOETHYLENIMINE TRANSFECTION EFFICIENCY HYPERBRANCHED PEI NONVIRAL VECTORS LOW TOXICITY PLASMID DNA IN-VIVO
ISSN号	1742-7061
通讯作者	tian h
中文摘要	to search for potential non-viral nucleic acids carriers, a series of novel cationic polymers, multi-armed poly(aspartate-graft-oligoethylenimine) (mp-g-oei) copolymers were designed and synthesized by grafting different types of oligoethylenimine (oei) to a multi-armed poly(l-aspartic acid) backbone. the as-synthesized mp-g-oei copolymers were characterized by fourier transform infrared spectroscopy, nuclear magnetic resonance and gel permeation chromatography. these mp-g-oei copolymers (mp423, mp600 and mp1800) exhibited good capacity in condensing nucleic acids (pdna or sirna) into nanosized particles (90-150 nm) with positive surface charges. gene transfection activity of the mp-g-oei copolymers (especially mp1800) showed improved performance compared with pei25k in both hela and cho cell lines. the silencing efficiency of mp600/sirna and mp1800/sirna complexes showed a superior knockdown effect in ct26 and huh-7 cell lines. moreover, the mp-g-oei copolymers exhibited much lower cytotoxicity than pei25k. flow cytometric analysis showed that mp-g-oei copolymers could efficiently mediate the entry of nucleic acids into cells. these results suggest that mp-g-oei copolymers may be potential non-viral gene carriers for the delivery of nucleic acids in future gene therapy. (c) 2013 acta materialia inc. published by elsevier ltd. all rights reserved.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000319951500021
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49828]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Dong X,Lin L,Chen J,et al. Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA[J]. acta biomaterialia,2013,9(6):6943-6952.
APA	Dong X.,Lin L.,Chen J.,Tian H.,Xiao C.,...&Chen X.(2013).Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA.acta biomaterialia,9(6),6943-6952.
MLA	Dong X,et al."Multi-armed poly(aspartate-g-OEI) copolymers as versatile carriers of pDNA/siRNA".acta biomaterialia 9.6(2013):6943-6952.