Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing

	Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing
	Wang Y ; Chen J ; Jiao HP ; Chen Y ; Li WY ; Zhang QF ; Yu C
刊名	chemistry-a european journal
	2013
卷号	19 期号:38 页码:12846-12852
关键词	ALKALINE-PHOSPHATASE ACTIVITY AGGREGATION-INDUCED EMISSION CONJUGATED POLYELECTROLYTES GOLD NANOPARTICLES ASSAY DNA BIOCHEMISTRY SENSORS SILOLE WATER
ISSN号	0947-6539
通讯作者	yu c
中文摘要	a new label-free fluorescence turn-on strategy for highly sensitive biosensing has been developed. a negatively charged perylene probe was synthesized. polycations could induce aggregation of the perylene probe through noncovalent interactions and the fluorescence of the probe's monomer was efficiently quenched. upon addition of a single-stranded nucleic acid, competitive binding of the negatively charged nucleic acid (a polyanion) to the cationic polymer resulted in the release of a monomer and thus a turn-on fluorescence signal was detected. without the use of any amplification techniques, a detection limit of 2pm dna was obtained. based on these results, an assay strategy for the highly sensitive detection of alkaline phosphatase (alp) activity has been demonstrated. exonuclease (exo) could degrade 5-phosphorylated single-stranded dna. however, when the dna sample was treated with alp, the phosphate functional group was removed by alp and it could no longer be degraded by exo. binding of the dna to the perylene probe-polycation complex resulted in a turn-on fluorescence signal, which could be used for sensing of alp. the method is highly sensitive, a limit of detection as low as 0.02muml(-1) alp was obtained. our method is simple, convenient, highly sensitive, and inexpensive.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000324316300039
公开日期	2014-04-15
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49612]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang Y,Chen J,Jiao HP,et al. Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing[J]. chemistry-a european journal,2013,19(38):12846-12852.
APA	Wang Y.,Chen J.,Jiao HP.,Chen Y.,Li WY.,...&Yu C.(2013).Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing.chemistry-a european journal,19(38),12846-12852.
MLA	Wang Y,et al."Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing".chemistry-a european journal 19.38(2013):12846-12852.