Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum
	Dzoyem, Jean P.1; NKuete, Antoine H. L.2; Kuete, Victor 1; Tala, Michel F.2; Wabo, Hippolyte K.2; Guru, Santosh K.3; Rajput, Vikrant S.4; Sharma, Akash 4; Tane, Pierre 2; Khan, Inshad A.4
刊名	PLANTA MEDICA
	2012-05-01
卷号	78 期号:8 页码:787-792
关键词	Polygonum limbatum Polygonaceae extract compounds antimicrobial cytotoxicity
ISSN号	0032-0943
通讯作者	Kuete, V (reprint author), Univ Dschang, Fac Sci, Dept Biochem, POB 67, Dschang 237, Cameroon,kuetevictor@yahoo.fr
英文摘要	The present study was designed to investigate the antimicrobial activity and the cytotoxicity of the methanol extract (PLA) as well as fractions (PLA1-4) and compounds [cardamomin (1), (+/-)-polygohomoisoflavanone (2), (S)-(-)-pinostrobin (3), 2',4'-dihydroxy-3',6'-dimethoxychalcone (4), (2S)-(-)-5-hydroxy-6,7-dimethoxyflavanone (5), and (2S)-(-)-5,7-dimethoxyflavanone (6)] obtained from leaves of Polygonum limbatum. The microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against 11 microbial strains including Candida albicans, C. krusei, C. tropicalis, Aspergillus fumigatus, Pseudomonas aeruginosa, Escherichia coli, vancomycin-resistant Enterococcus faecalis (VRE), Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S. epidermidis, and Mycobacterium tuberculosis H37Rv. The sulphorhodamine B cell growth inhibition assay was used to assess the cytotoxicity of the above samples on lung A549 adenocarcinoma, breast carcinoma MCF-7, prostate carcinoma PC-3, cervical carcinoma HeLa, and the acute monocytic leukemia cell line THP-1. The results of the MIC determination indicated that, apart from fraction PLA3, all other fractions as well as PLA and compound 3 were selectively active. MIC values were noted on 100% of the 11 tested microorganisms for fraction PLA3, 72.7% for PLA, fraction PLA2, and compound 4, 63.6% for PLA1, and 54.5% for fraction PLA4. The results of the cytotoxicity assay revealed that, except for A459 cells, more than 50% inhibition of the proliferation was obtained with each of the tested samples on at least one of the four other cell lines. IC50 values below 4 mu g/mL were obtained with 1 and 4 on THP-1 cells. The overall results of the present study provided baseline information for the possible use of Polygonum limbatum as well as some of the isolated compounds for the control of cancer diseases and mostly leukemia.
学科主题	Plant Sciences ; Pharmacology & Pharmacy
类目[WOS]	Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy
研究领域[WOS]	Plant Sciences ; Pharmacology & Pharmacy
关键词[WOS]	PHENOLIC-COMPOUNDS ; FLAVONOIDS ; PLANTS ; SENEGALENSE ; MEDICINE
收录类别	SCI
资助信息	The authors are thankful to Cameroon National Herbarium for the plant identification. JPD is thankful to Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) and the Centre for International Co-operation in Science (CICS) for three-month training fellowship in the Indian Institute of Integrative Medicine, Jammu. We also gratefully acknowledge financial support from the International Foundation for Science (IFS), Stockholm, Sweden, and the Organization for the Prohibition of Chemical Weapons, The Hague, Netherlands (IFS-OPCW, Grant No F/4901-1) to HKW and IFS-Grant F/4579-2 to VK. Financial support from the TWAS-CAS (The Academy of Sciences for the Developing World and The Chinese Academy of Sciences) through a travel grant to MFT at the Kunming Institute of Botany, China, is also acknowledged.
语种	英语
WOS记录号	WOS:000304449300007
公开日期	2014-04-02
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17621]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Univ Dschang, Fac Sci, Dept Biochem, Dschang 237, Cameroon 2.Univ Dschang, Fac Sci, Dept Chem, Dschang 237, Cameroon 3.Indian Inst Integrat Med, Canc Pharmacol Div, Cell Signalling Lab, Jammu, India 4.Indian Inst Integrat Med, Clin Microbiol Unit, Jammu, India 5.Univ Gottingen, Inst Organ & Biomol Chem, Gottingen, Germany 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Dzoyem, Jean P.,NKuete, Antoine H. L.,Kuete, Victor,et al. Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum[J]. PLANTA MEDICA,2012,78(8):787-792.
APA	Dzoyem, Jean P..,NKuete, Antoine H. L..,Kuete, Victor.,Tala, Michel F..,Wabo, Hippolyte K..,...&Tan, Ning-Hua.(2012).Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum.PLANTA MEDICA,78(8),787-792.
MLA	Dzoyem, Jean P.,et al."Cytotoxicity and Antimicrobial Activity of the Methanol Extract and Compounds from Polygonum limbatum".PLANTA MEDICA 78.8(2012):787-792.