Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国科学院东亚植物多样性与生物地理学重点实验室

	Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea
	Chen, Shaoxing 1,2; Wang, Chuanming 2; Xu, Jian-Ping 3; Yang, Zhu L.1
刊名	EXTREMOPHILES
	2014-03-01
卷号	18 期号:2 页码:195-206
关键词	Halorubrum Hypersaline environment Mobile genetic elements Horizontal gene transfer
ISSN号	1431-0651
通讯作者	Yang,ZL (reprint author),Chinese Acad Sci,Kunming Inst Bot,Key Lab Biodivers & Biogeog,Kunming 650201,Yunnan,Peoples R China. ; haloarchaea1979@gmail.com ; wcm_biology2@126.com ; fungi@mail.kib.ac.cn
英文摘要	A novel haloarchaeal plasmid, pHRDV1 (13,053 bp), was isolated from the haloarchaeal isolate Halorubrum sp. T3. Molecular and bioinformatics analyses showed that this element is a double-stranded circular DNA molecule containing two putative transcripts with opposite directions. The amino acid sequences of six of the nineteen predicted open reading frames were similar to those found in haloarchaeal pleomorphic viruses, such as Halorubrum pleomorphic virus 3 and Halogeometricum pleomorphic virus 1. There was also a strong conservation in gene order between the plasmid and these viruses. All three conserved viral proteins (VPs), which are characteristic of haloarchaeal pleomorphic viruses VP3, VP4 and VP8, were found in pHRDV1. Furthermore, a typical repressor-operator system similar to haloarchaeal myovirus phi Ch1, was found on the genome of pHRDV1. However, no viral particles were detected in the supernatants of Halorubrum sp. T3, either in the presence or absence of mitomycin C. These results imply that plasmid pHRDV1 is a distinctive virus-like mobile genetic element that harbors some unique properties that make it different from all of the known haloarchaeal plasmids or viruses.
学科主题	Biochemistry & Molecular Biology ; Microbiology
类目[WOS]	Biochemistry & Molecular Biology ; Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]	EXTREMELY HALOPHILIC ARCHAEA ; NUCLEOTIDE-SEQUENCE ; HOST INTERACTIONS ; MINIMAL REPLICON ; PLASMID ; HYPERSALINE ; GENOME ; ENVIRONMENTS ; IDENTIFICATION ; SULFOLOBUS
收录类别	SCI
资助信息	CAS/SAFEA International Partnership Program for Creative Research Teams; Hundred Talents Program of the Chinese Academy of Sciences
语种	英语
WOS记录号	WOS:000331970700001
公开日期	2014-04-09
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17951]
专题	昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Yunnan, Peoples R China 2.Honghe Univ, Univ Key Lab Crop High Qual & High Effect Cultiva, Mengzi, Peoples R China 3.McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada
推荐引用方式 GB/T 7714	Chen, Shaoxing,Wang, Chuanming,Xu, Jian-Ping,et al. Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea[J]. EXTREMOPHILES,2014,18(2):195-206.
APA	Chen, Shaoxing,Wang, Chuanming,Xu, Jian-Ping,&Yang, Zhu L..(2014).Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea.EXTREMOPHILES,18(2),195-206.
MLA	Chen, Shaoxing,et al."Molecular characterization of pHRDV1, a new virus-like mobile genetic element closely related to pleomorphic viruses in haloarchaea".EXTREMOPHILES 18.2(2014):195-206.