Biosynthesis of poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Biosynthesis of poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol
	Wang, Qi 1,3; Yang, Peng 1,4; Xian, Mo1,2 ; Yang, Ying 1; Liu, Changshui 1,3; Xue, Yongchang 4; Zhao, Guang 1,2
刊名	BIORESOURCE TECHNOLOGY
	2013-08-01
卷号	142 期号:2013 页码:741-744
关键词	Poly(3-hydroxypropionate-co-3-hydroxybutyrate) Controllable structure Copolymer Recombinant Escherichia coli
英文摘要	As the most representative biodegradable thermoplastic, poly(3-hydroxybutyrate) (P3HB) has a limited range of applications because of its poor thermal and physical properties. To improve its properties, a novel biosynthetic system was designed to produce poly(3-hydroxypropionate-co-3-hydroxybutyrate) (P(3HP-co-3HB)) with fully controllable structures from inexpensive carbon source. In this system, two parallel synthetic pathways controlled by independent regulatory systems were used to produce the 3HP and 3HB monomers, respectively. Through tuning the expression level of appropriate genes, P(3HP-co-3HB) copolyesters were synthesized with a wide range of 3HP fraction from 11.5 mol% to 94.6 mol%. Differential scanning calorimetry analysis demonstrated that the thermal properties of P(3HP-co-3HB) copolymer were totally dependent on its composition. The bioreactor cultivation was also performed and accumulated 9.8 g/L P(48.2 mol% 3HP-co-3HB) using glycerol as sole carbon source, which represented the highest production so far. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	COMONOMER COMPOSITIONAL DISTRIBUTION ; RECOMBINANT ESCHERICHIA-COLI ; MICROBIAL SYNTHESIS ; THERMAL-CHARACTERISTICS ; POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYPROPIONATE) ; POLYESTERS
收录类别	SCI
语种	英语
WOS记录号	WOS:000322292800103
公开日期	2014-03-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1672]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Biobased Mat, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qi,Yang, Peng,Xian, Mo,et al. Biosynthesis of poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol[J]. BIORESOURCE TECHNOLOGY,2013,142(2013):741-744.
APA	Wang, Qi.,Yang, Peng.,Xian, Mo.,Yang, Ying.,Liu, Changshui.,...&Zhao, Guang.(2013).Biosynthesis of poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol.BIORESOURCE TECHNOLOGY,142(2013),741-744.
MLA	Wang, Qi,et al."Biosynthesis of poly(3-hydroxypropionate-co-3-hydroxybutyrate) with fully controllable structures from glycerol".BIORESOURCE TECHNOLOGY 142.2013(2013):741-744.