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	Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method
	Song, Xiangfei1,2; Wang, Yefei1,2; Shu, Zhiyu1,2; Hong, Jingbo1,2; Li, Tong1,2; Yao, Lishan1,2
刊名	PLOS COMPUTATIONAL BIOLOGY
	2013-07-01
卷号	9期号:7
中文摘要	The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the Tm of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the method includes five steps, the single mutant computer screening to identify thermostable mutant candidates, the experimental evaluation to confirm the positive selections, the computational redesign around the thermostable mutation regions, the experimental reevaluation and finally the multiple mutations combination. The adopted method is simple and effective, can be applied to other important proteins where other methods have difficulties, and therefore provides a new tool to improve protein thermostability.
英文摘要	The ability to design thermostable proteins offers enormous potential for the development of novel protein bioreagents. In this work, a combined computational and experimental method was developed to increase the T-m of the flavin mononucleotide based fluorescent protein Bacillus Subtilis YtvA LOV domain by 31 Celsius, thus extending its applicability in thermophilic systems. Briefly, the method includes five steps, the single mutant computer screening to identify thermostable mutant candidates, the experimental evaluation to confirm the positive selections, the computational redesign around the thermostable mutation regions, the experimental reevaluation and finally the multiple mutations combination. The adopted method is simple and effective, can be applied to other important proteins where other methods have difficulties, and therefore provides a new tool to improve protein thermostability.
学科主题	仿真与模拟
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Mathematical & Computational Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Mathematical & Computational Biology
关键词[WOS]	GREEN FLUORESCENT PROTEIN ; FREE-ENERGY CALCULATIONS ; CHARGE-CHARGE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR-DYNAMICS ; REPORTER PROTEINS ; T4 LYSOZYME ; STABILITY ; STABILIZATION ; SIMULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000322320200017
公开日期	2014-03-21
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1634]
专题	青岛生物能源与过程研究所_仿真模拟团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Song, Xiangfei,Wang, Yefei,Shu, Zhiyu,et al. Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method[J]. PLOS COMPUTATIONAL BIOLOGY,2013,9(7).
APA	Song, Xiangfei,Wang, Yefei,Shu, Zhiyu,Hong, Jingbo,Li, Tong,&Yao, Lishan.(2013).Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method.PLOS COMPUTATIONAL BIOLOGY,9(7).
MLA	Song, Xiangfei,et al."Engineering a More Thermostable Blue Light Photo Receptor Bacillus subtilis YtvA LOV Domain by a Computer Aided Rational Design Method".PLOS COMPUTATIONAL BIOLOGY 9.7(2013).

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