Isolation and characterization of <i>Aurantiochytrium</i> species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, <i>Aurantiochytrium</i> sp. SD116

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	*Isolation and characterization of Aurantiochytrium* species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116**
	Gao, M. ; Song, X. ; Feng, Y. ; Li, W. ; & Cui, Q.
刊名	Journal of Oleo Science
	2013
卷号	62 期号:3 页码:143-151
关键词	Aurantiochytrium sp. biomass docosahexaenoic acid heterotrophic marine algae lipid
中文摘要	A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis of the 18S rDNA of SD116 showed that the strain has a close phylogenetic relationship to *Aurantiochytrium* species. The highest rates for growth and DHA accumulation for SD116 were obtained in 6.0% glucose, 2.0% yeast extract, and 50% artificial seawater (ASW) at a pH of 7 at 28℃. The maximum total lipid content reached 56.3% of the dry cell weight (DCW), and the maximum DHA content accounted for 50.9% of the total fatty acid (TFA) content. It was further found that urea may be a potential nitrogen source for industrial fermentation because of its cheap price and ability to induce a relatively high biomass and lipid production capacity. Using 5 L fermenters, the DCW, total lipid content, and DHA yield were found to be 70.43 g L－1, 71.09% of the DCW, and 17.42 g L－1 (34.79% of the TFA), respectively. The results show that *Aurantiochytrium* sp. SD116 is a promising candidate for commercial DHA production and could be useful for the synthesis of biomass-related products.
学科主题	代谢物组学
收录类别	SCI
语种	英语
公开日期	2014-03-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1675]
专题	青岛生物能源与过程研究所_代谢物组学团队
推荐引用方式 GB/T 7714	Gao, M.,Song, X.,Feng, Y.,et al. Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116[J]. Journal of Oleo Science,2013,62(3):143-151.
APA	Gao, M.,Song, X.,Feng, Y.,Li, W.,&& Cui, Q..(2013).Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116.Journal of Oleo Science,62(3),143-151.
MLA	Gao, M.,et al."Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga, Aurantiochytrium sp. SD116".Journal of Oleo Science 62.3(2013):143-151.