Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation

	Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation
	Li N(李宁); Mao DB(毛德斌); Gong YX(龚一心); Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
	2013
会议名称	World Congress on Medical Physics and Biomedical Engineering
会议日期	MAY 26-31, 2012
通讯作者邮箱	mlong@imech.ac.cn
会议地点	Beijing, China
关键词	LFA-1 Mac-1 ICAM-1 on-rate off-rate
卷号	39
页码	139-142
通讯作者	龙勉
中文摘要	LFA-1 and Mac-1, two β2 integrin members constitutively expressed on neutrophils, mediate leukocyte recruitment cascade by binding to the same ligand of ICAM-1. The slow rolling and firm adhesion of leukocytes rely on LFA-1 while the cell crawling is dependent on Mac-1. We hypothesized that their distinct roles are likely attributed to the differences in the binding kinetics or in the diverse responses of outside-in and inside-out signaling. In this study, we compared the ICAM-1 binding features between soluble or membrane-expressed LFA-1 and Mac-1 with different affinity conformation using optical trap technique. Our data indicated that the affinity up-regulation from wide type (WT) to high affinity (HA) is off-rate dependent for LFA-1 but on-rate dependent for Mac-1. The structural bases of this new finding were found to be consistent with our previous simulations. These results furthered our understanding in their function differences under shear flow.
收录类别	EI
产权排序	[Li,Ning;Mao,Debin;Gong,Yixin;Lü, Shouqin;Zhang,Yan;Long,Mian] Key Laboratory of Microgravity (National Microgravity Laboratory), Institute of Mechanics, Chinese Academy of Sciences;[Li,Ning;Mao,Debin;Gong,Yixin;Lü, Shouqin;Zhang,Yan;Long,Mian] Center of Biomechanics and Bioengineering, Institute of Mechanics, China
会议网址	http://link.springer.com/chapter/10.1007/978-3-642-29305-4_39
会议录	World Congress on Medical Physics and Biomedical Engineering
会议录出版者	Springer Verlag,Heidelberg
语种	英语
ISSN号	1680-0737
ISBN号	978-3-642-29304-7
内容类型	会议论文
源URL	[http://dspace.imech.ac.cn/handle/311007/48306]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Li N,Mao DB,Gong YX,et al. Distinct kinetics features of LFA-1 and Mac-1 in neutrophil activation[C]. 见:World Congress on Medical Physics and Biomedical Engineering. Beijing, China. MAY 26-31, 2012.http://link.springer.com/chapter/10.1007/978-3-642-29305-4_39.