Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor

	Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor
	Liu L(刘丽); Viallat A; Jin G(靳刚)
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2014-01
通讯作者邮箱	annie.viallat@inserm.fr; gajin@imech.ac.cn
卷号	190 页码:221-226
关键词	Giant lipid vesicles (GUVs) Vesicles adhesion Non-specific adhesion Total internal reflection imaging ellipsometry (TIRIE) Biosensor
ISSN号	0925-4005
通讯作者	Viallat, A (reprint author), Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France.
产权排序	[Liu, Li; Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; [Viallat, Annie] Aix Marseille Univ, CNRS, UMR 7333, INSERM,LAI,UMR 1067, F-13009 Marseille, France
合作状况	国际
中文摘要	The behavior of giant lipid vesicles (GUVs) interacting with an adhesive surface coated with poly-L-lysine is investigated by a biosensor based on total internal reflection imaging ellipsometry (TIRIE). The adhesion of GUVs on the adhesive surface is regulated by the concentration of the poly-L-lysine coating, the pH of the vesicle suspension, and the flow rate of the suspending fluid. The TIRIE biosensor detects very clearly and sensitively GUV adsorption, flattening, rupture, and de-adhesion, all these behaviors being independently observed by phase contrast microscopy. These results show that the setting of the TIRIE biosensor, optimized for molecular sensing can be extended to GUV sensing. This work paves the way to develop a new sensitive high-throughput, low-consumption and low-cost biosensor technology for micron-size objects such as cells, capsules and liposomes.
学科主题	交叉与边缘领域的力学
分类号	二类/Q1
收录类别	SCI ; EI
原文出处	http://dx.doi.org/10.1016/j.snb.2013.08.044
语种	英语
WOS记录号	WOS:000326687700029
公开日期	2014-02-13
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/48076]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Liu L,Viallat A,Jin G. Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor[J]. SENSORS AND ACTUATORS B-CHEMICAL,2014,190:221-226.
APA	刘丽,Viallat A,&靳刚.(2014).Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor.SENSORS AND ACTUATORS B-CHEMICAL,190,221-226.
MLA	刘丽,et al."Vesicle adhesion visualized with total internal reflection imaging ellipsometry biosensor".SENSORS AND ACTUATORS B-CHEMICAL 190(2014):221-226.