Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)

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	Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)
	Zhong, Chengrong 1,2; Song, Yanlong 1,2; Wang, Yaping 1; Zhang, Tanglin 1; Duan, Ming 1; Li, Yongming 1; Liao, Lanjie 1; Zhu, Zuoyan 1; Hu, Wei 1
刊名	GENERAL AND COMPARATIVE ENDOCRINOLOGY
	2013-10-01
卷号	192 页码:81-88
关键词	Growth hormone AgRP NPY Food intake Transgenic Common carp
ISSN号	0016-6480
通讯作者	Hu, W (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	In fish, food intake and feeding behavior are crucial for survival, competition, growth and reproduction. Growth hormone (GH)-transgenic common carp exhibit an enhanced growth rate, increased food intake and higher feed conversion rate. However, the underlying molecular mechanisms of feeding regulation in GH-transgenic (TG) fish are not clear. In this study, we observed feeding behavior of TG and non-transgenic (NT) common carp, and analyzed the mRNA expression levels of NPY, AgRP I, orexin, POMC, CCK, and CART I in the hypothalamus and telencephalon after behavioral observation. We detected similar gene expression levels in the hypothalamus of TG and NT common carp, which had been cultured in the field at the same age. Furthermore, we tested the effects of GH on hypothalamus fragments in vitro to confirm our findings. We demonstrated that TG common carp displayed increased food intake and reduced food consumption time, which were associated with a marked increase in hypothalamic AgRP I mRNA expression. Our results suggest that elevated GH levels may influence food intake and feeding behavior by upregulating the hypothalamic orexigenic factor AgRP I in GH-transgenic common carp. (C) 2013 Elsevier Inc. All rights reserved.
英文摘要	In fish, food intake and feeding behavior are crucial for survival, competition, growth and reproduction. Growth hormone (GH)-transgenic common carp exhibit an enhanced growth rate, increased food intake and higher feed conversion rate. However, the underlying molecular mechanisms of feeding regulation in GH-transgenic (TG) fish are not clear. In this study, we observed feeding behavior of TG and non-transgenic (NT) common carp, and analyzed the mRNA expression levels of NPY, AgRP I, orexin, POMC, CCK, and CART I in the hypothalamus and telencephalon after behavioral observation. We detected similar gene expression levels in the hypothalamus of TG and NT common carp, which had been cultured in the field at the same age. Furthermore, we tested the effects of GH on hypothalamus fragments in vitro to confirm our findings. We demonstrated that TG common carp displayed increased food intake and reduced food consumption time, which were associated with a marked increase in hypothalamic AgRP I mRNA expression. Our results suggest that elevated GH levels may influence food intake and feeding behavior by upregulating the hypothalamic orexigenic factor AgRP I in GH-transgenic common carp. (C) 2013 Elsevier Inc. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Endocrinology & Metabolism
研究领域[WOS]	Endocrinology & Metabolism
关键词[WOS]	SALMON ONCORHYNCHUS-KISUTCH ; NEUROPEPTIDE-Y NEURONS ; CCK/GASTRIN-LIKE IMMUNOREACTIVITY ; COHO SALMON ; ATLANTIC SALMON ; CARASSIUS-AURATUS ; FEEDING-BEHAVIOR ; MELANOCORTIN-4 RECEPTOR ; SWIMMING PERFORMANCE ; HYPOTHALAMIC ARCUATE
收录类别	SCI
资助信息	National Natural Science Foundation [30930069]; Development Plan of the State Key Fundamental Research of China [2010CB126302]; Key Research Program of the Chinese Academy of Sciences [KSCX2-EW-N-004]; '863' High Technology Project [2011AA100404]
语种	英语
WOS记录号	WOS:000325736400011
公开日期	2014-01-24
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19776]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhong, Chengrong,Song, Yanlong,Wang, Yaping,et al. Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2013,192:81-88.
APA	Zhong, Chengrong.,Song, Yanlong.,Wang, Yaping.,Zhang, Tanglin.,Duan, Ming.,...&Hu, Wei.(2013).Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP).GENERAL AND COMPARATIVE ENDOCRINOLOGY,192,81-88.
MLA	Zhong, Chengrong,et al."Increased food intake in growth hormone-transgenic common carp (Cyprinus carpio L.) may be mediated by upregulating Agouti-related protein (AgRP)".GENERAL AND COMPARATIVE ENDOCRINOLOGY 192(2013):81-88.