Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish

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	Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish
	Wang Yang; Zhou Li; Li Zhi; Li WenHua; Gui JianFang
刊名	SCIENCE CHINA-LIFE SCIENCES
	2013-11-01
卷号	56 期号:11 页码:975-984
关键词	epiboly apolipoprotein C1 E-cadherin radial intercalation
ISSN号	1674-7305
通讯作者	Gui, JF (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	Apolipoprotein C1 (Apoc1) is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoc1b, a transcript isoform of Apoc1, in epiboly during zebrafish gastrulation. Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoc1b by injecting antisense morpholino (MO) caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos. Therefore, Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.
英文摘要	Apolipoprotein C1 (Apoc1) is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoc1b, a transcript isoform of Apoc1, in epiboly during zebrafish gastrulation. Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoc1b by injecting antisense morpholino (MO) caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos. Therefore, Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	ORANGE-SPOTTED GROUPER ; CELL MOVEMENTS ; VERTEBRATE GASTRULATION ; MOLECULAR-CLONING ; E-CADHERIN ; EXPRESSION ; YOLK ; MICROFILAMENTS ; ORGANIZATION ; CYTOSKELETON
收录类别	SCI
资助信息	National Basic Research Program of China [2010CB126300]; National High Technology Research and Development Program of China [2012AA092201-3]; Ministry of Agriculture of China [200903045]; National Natural Science Foundation of China [30971414]
语种	英语
WOS记录号	WOS:000326813300001
公开日期	2014-01-24
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19772]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang Yang,Zhou Li,Li Zhi,et al. Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(11):975-984.
APA	Wang Yang,Zhou Li,Li Zhi,Li WenHua,&Gui JianFang.(2013).Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish.SCIENCE CHINA-LIFE SCIENCES,56(11),975-984.
MLA	Wang Yang,et al."Apolipoprotein C1 regulates epiboly during gastrulation in zebrafish".SCIENCE CHINA-LIFE SCIENCES 56.11(2013):975-984.