Optical detection of acetylcholine esterase based on CdTe quantum dots

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	Optical detection of acetylcholine esterase based on CdTe quantum dots
	Chen, ZZ ; Ren, XL ; Tang, FQ
刊名	CHINESE SCIENCE BULLETIN
	2013
卷号	58 期号:21 页码:2622
关键词	quantum dots fluorescence detection acetylcholine esterase
ISSN号	1001-6538
通讯作者	Tang, FQ (reprint author), Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, Beijing 100190, Peoples R China.
中文摘要	In this study, we have constructed a simple, sensitive and rapid biosensor for detection of acetylcholine esterase (AChE) based on CdTe quantum dots (QDs). The detection limit of AChE by one-step enzyme reaction based on the thiolglycolic acid (TGA) stabilized QDs (TGA-QDs) was 10 U/L and the linear range was 10-100 and 100-1200 U/L, respectively. The detection limit of AChE by two-step enzyme reaction based on the 3-mercaptopropionic acid (MGA) stabilized QDs (MGA-QDs) was found to be 20 U/L and the linear range was 100-2500 U/L. The experimental conditions of biosensors were optimized, and the detection mechanism was studied. We also detected AChE in serum samples based on TGA-QDs or MGA-QDs. The linear range was 10-140 and 50-1000 U/L, respectively. The excellent performance of this novel biosensor demonstrated that this strategy has prodigious potential to be applied in practice detection of AChE.
资助信息	National Natural Science Foundation of China [81171454, 61178035]
语种	英语
公开日期	2014-01-16
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/57291]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Chen, ZZ,Ren, XL,Tang, FQ. Optical detection of acetylcholine esterase based on CdTe quantum dots[J]. CHINESE SCIENCE BULLETIN,2013,58(21):2622.
APA	Chen, ZZ,Ren, XL,&Tang, FQ.(2013).Optical detection of acetylcholine esterase based on CdTe quantum dots.CHINESE SCIENCE BULLETIN,58(21),2622.
MLA	Chen, ZZ,et al."Optical detection of acetylcholine esterase based on CdTe quantum dots".CHINESE SCIENCE BULLETIN 58.21(2013):2622.