Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump

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	Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump
	Zhang, QL ; Wang, L ; Weng, YX ; Qiu, XG ; Wang, WC ; Yan, JX
刊名	CHINESE PHYSICS
	2005
卷号	14 期号:12 页码:2484
关键词	FAST EVENTS DYNAMICS PEPTIDE SYSTEMS RANGE
ISSN号	1009-1963
通讯作者	Zhang, QL (reprint author), Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100080, Peoples R China.
中文摘要	Elucidating the initial kinetics of folding pathways is critical to the understanding of the protein folding mechanism. Transient infrared spectroscopy has proved a powerful tool to probe the folding kinetics. Herein we report the construction of a nanosecond laser-induced temperature-jump (T-jump) technique coupled to a nanosecond time-resolved transient mid-infrared (mid-IR) spectrometer system capable of investigating the protein folding kinetics with a temporal resolution of 50 ns after deconvolution of the instrumental response function. The mid-IR source is a liquid N-2 cooled CO laser covering a spectral range of 5.0 mu m (2000 cm(-1)) similar to 6.5 mu m (1540 cm(-1)). The heating pulse was generated by a high pressure H-2 Raman shifter at wavelength of 1.9 mu m. The maximum temperature-jump could reach as high as 26 +/- 1 degrees C. The fast folding/unfolding dynamics of cytochrome C was investigated by the constructed system, providing an example.
收录类别	SCI
语种	英语
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/49691]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Zhang, QL,Wang, L,Weng, YX,et al. Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump[J]. CHINESE PHYSICS,2005,14(12):2484.
APA	Zhang, QL,Wang, L,Weng, YX,Qiu, XG,Wang, WC,&Yan, JX.(2005).Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump.CHINESE PHYSICS,14(12),2484.
MLA	Zhang, QL,et al."Nanosecond-time-resolved infrared spectroscopic study of fast relaxation kinetics of protein folding by means of laser-induced temperature-jump".CHINESE PHYSICS 14.12(2005):2484.