An improved single molecule manipulation apparatus and its applications

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	An improved single molecule manipulation apparatus and its applications
	Wang, XL ; Zhang, XH ; Wei, KJ ; Sun, B ; Li, M
刊名	ACTA PHYSICA SINICA
	2008
卷号	57 期号:6 页码:3905
关键词	COLI RNA-POLYMERASE SUPERCOILED DNA TRANSLOCATION TRANSCRIPTION ELASTICITY TENSION FORCE FTSK
ISSN号	1000-3290
通讯作者	Li, M: Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, POB 603, Beijing 100080, Peoples R China.
中文摘要	We report an improvement to the lateral-magnetic-tweezers which can be used to stretch and twist DNA molecules, and to probe the interaction between DNA and proteins. The apparatus enables not only direct measurement of the extension and rotation of DNA but also easy refreshing of the buffer in the flow chamber. With this apparatus, the experimental efficiency can be greatly improved by manipulating several DNA molecules simultaneously. The force exerted on DNA molecules ranges from 0.1 to 40 pN, satisfying the requirement of most single molecule experiments. To demonstrate the applicability of the apparatus, we measured the extension-force curve and the extensions rotation curve of DNA and studied the translocation of DNA by a motor protein. The results agree well with those reported in the literature.
收录类别	SCI
语种	中文
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/33653]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Wang, XL,Zhang, XH,Wei, KJ,et al. An improved single molecule manipulation apparatus and its applications[J]. ACTA PHYSICA SINICA,2008,57(6):3905.
APA	Wang, XL,Zhang, XH,Wei, KJ,Sun, B,&Li, M.(2008).An improved single molecule manipulation apparatus and its applications.ACTA PHYSICA SINICA,57(6),3905.
MLA	Wang, XL,et al."An improved single molecule manipulation apparatus and its applications".ACTA PHYSICA SINICA 57.6(2008):3905.