A procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique

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	A procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique
	Zheng, XF ; Gu, YX ; Zheng, CD ; Mo, YD ; Fan, HF ; Hao, Q
刊名	ZEITSCHRIFT FUR KRISTALLOGRAPHIE
	1997
卷号	212 期号:2 页码:95
关键词	CRYSTAL-STRUCTURE ISOMORPHOUS REPLACEMENT RESOLUTION
ISSN号	0044-2968
中文摘要	Both direct methods and the solvent flattening technique have been proved capable of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (GAS) data. However, in theory, while direct methods are better in deriving starting phases from OAS data, the solvent flattening technique is more powerful in the subsequent phase refinement. A procedure is described in this paper which combines direct methods with the solvent flattening technique for phasing of OAS data. The procedure has been tested with the known protein RNase Sa. This protein is of moderate size, and the diffraction data were collected routinely without taking special care for the recording of Friedels. The test proved that the combination of the direct method and the solvent flattening leads to a much better result than that from either method alone.
收录类别	SCI
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/33184]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Zheng, XF,Gu, YX,Zheng, CD,et al. A procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique[J]. ZEITSCHRIFT FUR KRISTALLOGRAPHIE,1997,212(2):95.
APA	Zheng, XF,Gu, YX,Zheng, CD,Mo, YD,Fan, HF,&Hao, Q.(1997).A procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique.ZEITSCHRIFT FUR KRISTALLOGRAPHIE,212(2),95.
MLA	Zheng, XF,et al."A procedure for ab initio phasing of one-wavelength anomalous scattering data from proteins - Combining direct methods with the solvent flattening technique".ZEITSCHRIFT FUR KRISTALLOGRAPHIE 212.2(1997):95.