Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage
	Zhao, Longyan1,2 ; Lai, Sensen 1,3,4; Huang, Rong 5; Wu, Mingyi 1; Gao, Na 1; Xu, Li 1,2; Qin, Hongbo1 ; Peng, Wenlie 3; Zhao, Jinhua1
刊名	CARBOHYDRATE POLYMERS
	2013-11-06
卷号	98 期号:2 页码:1514-1523
关键词	Sea cucumber Glycosaminoglycan Deacetylation Deaminative cleavage Anticoagulant activity
ISSN号	0144-8617
通讯作者	Qin,HB (reprint author),Chinese Acad Sci,Kunming Inst Bot,State Key Lab Phytochem & Plant Resources West Ch,Kunming,Peoples R China.,qinhongbo@mail.kib.ac.cn ; peng-wenlie@21cn.com
英文摘要	Fucosylated glycosaminoglycans (FGs) are complex glycosaminoglycans that exhibit potent anticoagulant activity. To study the relationship between molecular size and biological activity, oligosaccharides with (2,5)-anhydro-D-talose units at new reducing ends were prepared by hydrazine deacetylation and nitrous acid depolymerization. The product chemical structures were analyzed by one- and two-dimensional NMR methods. Additionally, anticoagulant activities were evaluated by clotting assay and chromogenic substrate cleavage. The results demonstrated that under mild deacetylation and deaminative cleavage conditions, both products were relatively homogeneous and sulfated fucose branch types and sulfate sub-stituents remained stable. These depolymerized FGs with different molecular sizes had potent intrinsic anticoagulant activities, which were similar to those that were obtained by free-radical depolymerization with similar molecular weights. Decreasing molecular weight may weaken activity but not significantly affect factor Xase and heparin cofactor II (HCII)-mediated thrombin inhibition. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Chemistry ; Polymer Science
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN ; NITROUS-ACID DEPOLYMERIZATION ; FREE-RADICAL DEPOLYMERIZATION ; STICHOPUS-JAPONICUS SELENKA ; CUCUMBER THELENATA-ANANAS ; CHONDROITIN SULFATE ; SEA-CUCUMBER ; PHYSICOCHEMICAL CHARACTERISTICS ; HEPARIN ; OLIGOSACCHARIDES
收录类别	SCI
资助信息	Yunnan Provincial Science and Technology Department in China [2010CI116, 2012BC011, 2012FB177];National Natural Science Foundation of China [81102372];Outstanding Technical Talent Foundation of the Chinese Academy of Sciences;West Light Foundation of the Chinese Academy of Sciences
语种	英语
WOS记录号	WOS:000326313600035
公开日期	2014-01-13
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17420]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Sun Yat Sen Univ, Coll Life Sci, Guangzhou 510275, Guangdong, Peoples R China 4.Ganzhou Prov Inst Food & Drug Control, Ganzhou, Peoples R China 5.Yunan Univ, Sch Chem Sci & Technol, Minist Educ, Key Lab Med Chem Nat Resource, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Longyan,Lai, Sensen,Huang, Rong,et al. Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage[J]. CARBOHYDRATE POLYMERS,2013,98(2):1514-1523.
APA	Zhao, Longyan.,Lai, Sensen.,Huang, Rong.,Wu, Mingyi.,Gao, Na.,...&Zhao, Jinhua.(2013).Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage.CARBOHYDRATE POLYMERS,98(2),1514-1523.
MLA	Zhao, Longyan,et al."Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage".CARBOHYDRATE POLYMERS 98.2(2013):1514-1523.