Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)
	Zhu, Chuankun 1,2; Yu, Xiaomu 1; Fu, B.1; Tong, Jingou 1
刊名	CONSERVATION GENETICS RESOURCES
	2013-09-01
卷号	5 期号:3 页码:755-758
关键词	Bighead carp (Aristichthys nobilis) Microsatellite Polymorphism Silver carp (Hypophthalmichthys molitrix) Cross-species amplification
ISSN号	1877-7252
通讯作者	Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp.
英文摘要	In this study we developed 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis), and 135 of them showed polymorphism. Thirty-seven of polymorphic loci were randomly selected to characterize genetic diversity in a test population with 36 individuals. The number of alleles per locus ranged from 2 to 11 (mean 5.6), and the average observed and expected heterozygosity were 0.53 and 0.61, respectively. Twenty-four of the 37 loci were highly informative (PIC > 0.5) in the test population. Six loci deviated from the Hardy-Weinberg Equilibrium, and no pairs of loci were in linkage disequilibrium after Bonferroni correction. All 201 loci could be successfully amplified in silver carp (Hypophthalmichthys molitrix) with 64 loci being polymorphic, indicating a high universality of these microsatellites across species. These novel polynucleotide microsatellites would be useful tools for further population and conservation genetics studies in bighead carp and its closely-related silver carp.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biodiversity Conservation ; Genetics & Heredity
研究领域[WOS]	Biodiversity & Conservation ; Genetics & Heredity
收录类别	SCI
资助信息	MOA [200903045]; NSFC [31272647]; MOST 973 [2010CB126305]; TGC [CT-12-08-01]; FEBL of China [2011FBZ20]
语种	英语
WOS记录号	WOS:000322619900037
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19578]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Chuankun,Yu, Xiaomu,Fu, B.,et al. Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)[J]. CONSERVATION GENETICS RESOURCES,2013,5(3):755-758.
APA	Zhu, Chuankun,Yu, Xiaomu,Fu, B.,&Tong, Jingou.(2013).Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis).CONSERVATION GENETICS RESOURCES,5(3),755-758.
MLA	Zhu, Chuankun,et al."Development of 201 tri- and tetra-nucleotide repeat microsatellites for bighead carp (Aristichthys nobilis)".CONSERVATION GENETICS RESOURCES 5.3(2013):755-758.