Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR

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	Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR
	Li, Huiying; Cai, Yan; Xie, Ping; Li, Guangyu; Hao, Le; Xiong, Qian
刊名	ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
	2013-10-01
卷号	65 期号:3 页码:537-545
关键词	INTERLEUKIN-8 GENE-EXPRESSION PRO-INFLAMMATORY CYTOKINES TROUT ONCORHYNCHUS-MYKISS 3'-UNTRANSLATED REGION REAL-TIME IN-VIVO CHEMOKINES IL-1-BETA CLONING MACROPHAGES
ISSN号	0090-4341
通讯作者	Xie, P (reprint author), Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
英文摘要	Microcystin-LR (MCLR) is a widespread cyanotoxin and has immunotoxicity to animals, including fish. Chemokines are considered to play important roles in inflammatory response induced by MCLR. In this study, we cloned the full-length cDNA of interleukin-8 (IL-8) from bighead carp (Aristichthys nobilis) for the first time. The full-length IL-8 cDNA was 552 bp and contained a 297-bp open-reading frame that encoded for a 98-amino acid protein. The deduced IL-8 protein had a typical aspartic acid (D)-leucine (L)-arginine (R) and a CXC motif at the N-terminal, which were conserved in most fish species. Phylogenetic analysis showed that bighead carp IL-8 protein was grouped in the teleost IL-8 lineage 2. Under normal conditions, the expression of IL-8 is constitutive and weak in all tested tissues. However, MCLR treatment could significantly increase the transcription of IL-8 in bighead carp in a temporal- and dose-dependent pattern. The present study will help us to understand more about the evolution of IL-8 and its function in the MCLR induced proinflammatory response in bighead carp.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Environmental Sciences ; Toxicology
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
关键词[WOS]	INTERLEUKIN-8 GENE-EXPRESSION ; PRO-INFLAMMATORY CYTOKINES ; TROUT ONCORHYNCHUS-MYKISS ; 3'-UNTRANSLATED REGION ; REAL-TIME ; IN-VIVO ; CHEMOKINES ; IL-1-BETA ; CLONING ; MACROPHAGES
收录类别	SCI
资助信息	National Natural Science Foundation of China [31070457]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ01]
语种	英语
WOS记录号	WOS:000324074200017
公开日期	2014-01-03
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19543]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Li, Huiying,Cai, Yan,Xie, Ping,et al. Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR[J]. ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2013,65(3):537-545.
APA	Li, Huiying,Cai, Yan,Xie, Ping,Li, Guangyu,Hao, Le,&Xiong, Qian.(2013).Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR.ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,65(3),537-545.
MLA	Li, Huiying,et al."Identification and Expression Profiles of IL-8 in Bighead Carp (Aristichthys nobilis) in Response to Microcystin-LR".ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 65.3(2013):537-545.