Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives

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	Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives
	Liu, Tao 1,2; Zhang, Guifeng 1; Li, Suping 1,2; Wang, Yinjue 1,2; Ma, Guanghui 1; Su, Zhiguo 1
刊名	ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
	2011-02-01
卷号	39 期号:1 页码:19-25
关键词	gelatin plasma substitute colloid elimination rate half life high performance liquid chromatography
ISSN号	1073-1199
其他题名	Artif. Cells Blood Substit. Biotechnol.
中文摘要	The gelatin plasma substitute is often polydisperse and heterogenous, making it difficult to determine the elimination rate and half-life in the body. In this study, one method was developed based on quantitative determination of hydroxyproline derivatives. Two plasma substitutes were prepared by succinylation and genipin-crosslinking, respectively. After transfusion, the blood samples were hydrolyzed and derivatized, and then analyzed by HPLC. A two-phase exponential association equation was used for fitting the time-concentration curves. The results indicated that this method could be used for quantitative determination of gelatin in blood, and the pharmacokinetic parameters such as elimination rate and half-life.
英文摘要	The gelatin plasma substitute is often polydisperse and heterogenous, making it difficult to determine the elimination rate and half-life in the body. In this study, one method was developed based on quantitative determination of hydroxyproline derivatives. Two plasma substitutes were prepared by succinylation and genipin-crosslinking, respectively. After transfusion, the blood samples were hydrolyzed and derivatized, and then analyzed by HPLC. A two-phase exponential association equation was used for fitting the time-concentration curves. The results indicated that this method could be used for quantitative determination of gelatin in blood, and the pharmacokinetic parameters such as elimination rate and half-life.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]	LIGHT-SCATTERING ; CAPILLARY-ELECTROPHORESIS ; GENIPIN ; COLLAGEN ; RATS ; QUANTITATION ; METABOLISM ; COLLOIDS ; PROTEIN ; MODEL
收录类别	SCI
原文出处	://WOS:000286938300005
语种	英语
WOS记录号	WOS:000286938300005
公开日期	2013-11-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6267]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Tao,Zhang, Guifeng,Li, Suping,et al. Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2011,39(1):19-25.
APA	Liu, Tao,Zhang, Guifeng,Li, Suping,Wang, Yinjue,Ma, Guanghui,&Su, Zhiguo.(2011).Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,39(1),19-25.
MLA	Liu, Tao,et al."Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 39.1(2011):19-25.