Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides

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	Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides
	Liu, Qiao-Ning 1; Liu, Rui-Sang 1; Wang, Yi-Hua 2; Mi, Zhi-Yuan 1; Li, Dong-Sheng 1; Zhong, Jian-Jiang 3; Tang, Ya-Jie 1,4
刊名	BIORESOURCE TECHNOLOGY
	2009-07-01
卷号	100 期号:14 页码:3644-3649
关键词	Medicinal mushroom Tuber melanosporum Fed-batch fermentation Tuber polysaccharides
ISSN号	0960-8524
通讯作者	Tang, YJ
英文摘要	For the first time, a fed-batch fermentation process of Tuber melanosporum was developed for the efficient production of bioactive mycelia and Tuber polysaccharides. Each 1.67 g/L of peptone and 8.33 g/L of yeast extract were added on day 3, 6, and 9, respectively, and sucrose was fed to maintain its concentration around 35-5 g/L when its residual level decreased to 10-5 g/L. Then, the maximal biomass, the production of extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) reached 53.72 +/- 2.57 g DW/L, 7.09 +/- 0.62 and 4.43 +/- 0.21 g/L, respectively. Compared with the batch culture conducted in the enriched medium, the biomass, the production of EPS and IPS were enhanced by 55.8%, 222.3% and 103.2%, respectively. Not only the cell density but also the production of EPS and IPS were the highest ever reported in truffle fermentation, and the biomass was also the highest as ever reported in mushroom fermentation. (C) 2009 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]	SUBMERGED CULTIVATION ; CELL-GROWTH ; CULTURE ; SINENSE ; EXOPOLYSACCHARIDES ; CORDYCEPIN ; MUSHROOMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000266153700030
公开日期	2013-12-06
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/6638]
专题	过程工程研究所_多相复杂系统国家重点实验室
作者单位	1.Hubei Univ Technol, Coll Bioengn, Key Lab Fermentat Engn Minister Educ, Hubei Prov Key Lab Ind Microbiol, Wuhan 430068, Peoples R China 2.Jiangxi Univ Agr, Coll Sci, Nanchang 330045, Peoples R China 3.Shanghai Jiao Tong Univ, Key Lab Microbial Metab, Minist Educ, Coll Life Sci & Biotechnol, Shanghai 200240, Peoples R China 4.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qiao-Ning,Liu, Rui-Sang,Wang, Yi-Hua,et al. Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides[J]. BIORESOURCE TECHNOLOGY,2009,100(14):3644-3649.
APA	Liu, Qiao-Ning.,Liu, Rui-Sang.,Wang, Yi-Hua.,Mi, Zhi-Yuan.,Li, Dong-Sheng.,...&Tang, Ya-Jie.(2009).Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides.BIORESOURCE TECHNOLOGY,100(14),3644-3649.
MLA	Liu, Qiao-Ning,et al."Fed-batch fermentation of Tuber melanosporum for the hyperproduction of mycelia and bioactive Tuber polysaccharides".BIORESOURCE TECHNOLOGY 100.14(2009):3644-3649.