Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography
	Sun JN(孙建楠)1,2,3 ; Shi YP(师彦平)1,2 ; Chen J(陈娟)1,2
刊名	Analytical Methods
	2013
卷号	5 期号:6 页码:1427-1434
ISSN号	1759-9660
通讯作者	陈娟
英文摘要	Temperature controlled ionic liquid dispersive liquid-liquid microextraction (TC IL-DLLME) combined with high performance liquid chromatography (HPLC) has been developed for the simultaneous determination of di(2-ethylhexyl)phthalate (DEHP) and its metabolite mono(2-ethylhexyl) phthalate (MEHP) in human urine samples. Dibutyl phthalate (DBP) was employed as an internal standard. Some important parameters, such as the type of extraction solvent and dispersive solvent, the volume ratio of extraction to dispersive solvent, volume of a mixture of extraction solvent and dispersive solvent, temperature, sample pH, salt effect, cooling time and centrifugation time, were investigated and optimized to achieve a high extraction efficiency. Under the optimized extraction conditions, the method showed 115- and 164-fold enrichment factors for MEHP and DEHP in urine samples, respectively. It also exhibited high sensitivity with the limits of detection at 0.96 mu g L-1 and 3.2 mu g L^-1 for MEHP and DEHP, respectively. The calibration curves were linear in the concentration range of 20-1920 mu g L^-1 for MEHP and 60-1920 mu g L^-1 for DEHP, with the correlation coefficients higher than 0.9990. The proposed method can be expected to monitor the human exposure to the plasticizer agent by determining its metabolites in human urine samples.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Nature Science Foundation of China (no. 21075127 and 21105106);Nature Science Foundation of Gansu Province (no. 1107RJZA146)
语种	英语
WOS记录号	WOS:000315508900008
公开日期	2013-12-10
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/4585]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Sun JN,Shi YP,Chen J. Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography[J]. Analytical Methods,2013,5(6):1427-1434.
APA	Sun JN,Shi YP,&Chen J.(2013).Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography.Analytical Methods,5(6),1427-1434.
MLA	Sun JN,et al."Simultaneous determination of plasticizer di(2-ethylhexyl)phthalate and its metabolite in human urine by temperature controlled ionic liquid dispersive liquid-liquid microextraction combined with high performance liquid chromatography".Analytical Methods 5.6(2013):1427-1434.