Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study
	Chen J(陈娟)1 ; Jin L(晋玲)2; Li YD(李应东)2; Shi YP(师彦平)1
刊名	Analytical Methods
	2013
卷号	5 期号:24 页码:7058-7065
ISSN号	1759-9660
通讯作者	师彦平
英文摘要	The newly proposed strategy, namely, holistic analysis of multiple constituents from every constituent herb in a single run, was applied for the quality control of traditional Chinese medicine preparation Wuhu powder. Seven bioactive components, i.e., ferulic acid, hydroxysafflor yellow A, prim-O-glucosylcimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, imperatorin, isoimperatorin, and linoleic acid, from 5 medicinal herbs composing Wuhu powder were selected as evaluation markers, and then their quantitative information was obtained in a single run by an UPLC method coupled with photodiode array detection. Optimum separation was achieved on a C₁₈ column (50 × 2.1 mm i.d., 1.7 μm particle) through a gradient delivery of a mixture of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.3 mL min^_¹ at 30 ℃. The corresponding maximum absorption wavelengths were selected as the detection wavelengths for each analyte. For all of the analytes, a good linear relationship (r of >0.999) was obtained between peak area and concentration. The method was validated for repeatability, precision, stability, accuracy, and selectivity. The RSD values of retention time and peak area were 0–0.6% and 0.7–1.0% for intra-assay, and 0.1–1.0% and 1.6–1.8% for inter-assay precision. Analysis repeatability and stability (24 h) were all lower than 5.0%. The recovery of the method was 96.1–97.7% with a RSD of 2.4–4.0%. The validated method was applied to quality control of Wuhu powder from different production batches.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Key Technology Research and Development Program of China (no. 2011BAI05B02);the National Natural Science Foundation of China (no. 21105106)
语种	英语
WOS记录号	WOS:000327506600026
公开日期	2013-12-10
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/4584]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 2.Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Chen J,Jin L,Li YD,et al. Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study[J]. Analytical Methods,2013,5(24):7058-7065.
APA	Chen J,Jin L,Li YD,&Shi YP.(2013).Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study.Analytical Methods,5(24),7058-7065.
MLA	Chen J,et al."Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study".Analytical Methods 5.24(2013):7058-7065.