The Study on The Solution Structure of Full Length Primase From Bacillus subtilis

	The Study on The Solution Structure of Full Length Primase From Bacillus subtilis
	Luo Hao 1; Liu WenLin 1; Zhou YingQin 1; Tao Mei 1; Liu ZhongChuan 1; Wang GangGang 1
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2019
卷号	46 期号:11 页码:1101-1109
关键词	HELICASE-BINDING DOMAIN SMALL-ANGLE SCATTERING REPLICATION INITIATION DNAG STEAROTHERMOPHILUS CRYSTAL DNA replication primase SAXS flexibility
ISSN号	1000-3282
其他题名	The Study on The Solution Structure of Full Length Primase From Bacillus subtilis
英文摘要	In bacterial DNA replication, DnaG primase synthesizes RNA primers which are then extended by DNA polymerase. The DnaG primase consists of three domains, N-terminal zinc-binding domain (ZBD), RNA polymerase domain (RPD) and C-terminal helicase binding domain (HBD). In the process of producing primers, the three domains of primase cooperate with each other, and none is dispensable. Although the structures of the primase domains have been reported, so far, the full-length structure of the primase is not known yet. Here, the model of full-length DnaG in Bacillus subtilis (BsuDnaG) was constructed from the data of X-ray small angle scattering (SAXS) analysis. The BsuDnaG is in extended state in solution. On the other hand, the ZBD and HBD domains could exhibit continuous conformational changes relative to the RPD domain. This study suggests the domains rearrangement in DnaG primase may facilitate its function in DNA replication.
语种	英语
CSCD记录号	CSCD:6607298
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/35041]
专题	中国科学院烟台海岸带研究所
作者单位	1.中国科学院 2.中国科学院烟台海岸带研究所 3.中国科学院大学
推荐引用方式 GB/T 7714	Luo Hao,Liu WenLin,Zhou YingQin,et al. The Study on The Solution Structure of Full Length Primase From Bacillus subtilis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2019,46(11):1101-1109.
APA	Luo Hao,Liu WenLin,Zhou YingQin,Tao Mei,Liu ZhongChuan,&Wang GangGang.(2019).The Study on The Solution Structure of Full Length Primase From Bacillus subtilis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,46(11),1101-1109.
MLA	Luo Hao,et al."The Study on The Solution Structure of Full Length Primase From Bacillus subtilis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 46.11(2019):1101-1109.