Comparative genomic and protein sequence analyses of the chemotaxis system of Azorhizobium caulinodans

	Comparative genomic and protein sequence analyses of the chemotaxis system of Azorhizobium caulinodans
	Jiang Nan; Liu Wei; Li Yan; Xie Zhihong
刊名	Acta Microbiologica Sinica
	2016
卷号	56 期号:8 页码:1256-1265
关键词	趋化性甲基化受体蛋白茎瘤固氮根瘤菌
ISSN号	0001-6209
其他题名	Comparative genomic and protein sequence analyses of the chemotaxis system of Azorhizobium caulinodans
英文摘要	Objective Azorhizobium caulinodans ORS571 can fix nitrogen not only as a free-living organism and an associative-symbiotic bacterium by colonizing the root surface of non-leguminous plants, but also as a symbiotic bacterium by interacting with leguminous plant Sesbania rostrata. Due to its ability to grow and fix nitrogen under three conditions, A. caulinodans uses sophisticated chemotaxis signal transduction systems to transform environmental cues into corresponding behavioral responses. Chemotaxis appears crucial for the growth of A. caulinodansin complicated environment and the construction of associative relationship with the plant. However, little is known about the chemotactic pathway of A. caulinodans. Thus, our study aimed to compare the chemotaxis-like genes of A. caulinodans with those of well-studied species. Methods NCBI protein BLAST was used for searching sequence similarity with default parameter values against the genomes of A. caulinodans. HMMER3, based on Pfam database, was used for comparative analyses of methyl-accepting chemotaxis protein (MCP). Results There was a major chemotaxis cluster in A. caulinodans and the CheR methylated MCPs independently of pentapeptide motif. There were 43 MCP homologs containing diverse signal-sensing architectures in A. caulinodans. In addition, cytoplasmic domains of these MCPs were all composed of 38 heptad repeats. Conclusion Despite the extremely high homology presented between the chemotactic system of A. caulinodans and those of well-studied species, A. caulinodans shows its own unique characteristics. The classification of these chemotactic pathways by comparative genomics enables us to better understand how A. caulinodansresponds to changes in environment via exquisite signal transductions in chemotaxis system.
语种	英语
CSCD记录号	CSCD:5758285
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/35025]
专题	中国科学院烟台海岸带研究所
作者单位	中国科学院烟台海岸带研究所
推荐引用方式 GB/T 7714	Jiang Nan,Liu Wei,Li Yan,et al. Comparative genomic and protein sequence analyses of the chemotaxis system of Azorhizobium caulinodans[J]. Acta Microbiologica Sinica,2016,56(8):1256-1265.
APA	Jiang Nan,Liu Wei,Li Yan,&Xie Zhihong.(2016).Comparative genomic and protein sequence analyses of the chemotaxis system of Azorhizobium caulinodans.Acta Microbiologica Sinica,56(8),1256-1265.
MLA	Jiang Nan,et al."Comparative genomic and protein sequence analyses of the chemotaxis system of Azorhizobium caulinodans".Acta Microbiologica Sinica 56.8(2016):1256-1265.