Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber

doi:10.1007/s00343-023-3106-5

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber
	Li, Yanan 4; Chen, Zongfu 4; Zhang, Haibin 3; Liu, Ruoyu 2; Chen, Shuichun 1; Lin, Li 4
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2024-03-06
页码	12
关键词	Holothuroidea environmental adaptation positive selection point mutation
ISSN号	2096-5508
DOI	10.1007/s00343-023-3106-5
英文摘要	Manganese superoxide dismutase (MnSOD) is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria. In a dark, high-pressure, and low-temperature deep-sea environment, MnSOD is essential for the survival of sea cucumbers. Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers. To explore their environmental adaptation mechanism, we conducted environmental selection pressure analysis through the branching site model of PAML software. We obtained night positive selection sites, and two of them were significant (97F -> H, 134K -> V): 97F -> H located in a highly conservative characteristic sequence, and its polarity change might have a great impact on the function of MnSOD; 134K -> V had a change in piezophilic ability, which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea. To further study the effect of these two positive selection sites on MnSOD, we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL. Results show that 97F -> H, 134K -> V might improve MnSOD's efficiency of scavenging superoxide anion and its ability to resist high hydrostatic pressure by moderately reducing its stability. The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity, piezophilic behavior, and local stability. This study revealed the correlation between MnSOD and extreme environment, and will help improve our understanding of the organism's adaptation mechanisms in deep sea.
资助项目	Guangdong Province Basic and Applied Basic Research Fund Project[2020A1515110826] ; National Natural Science Foundation of China[42006115] ; Major Scientific and Technological Projects of Hainan Province[ZDKJ2021036]
WOS关键词	PHYLOGENETIC ANALYSIS ; MUTATIONS ; COPPER
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:001178950400001
资助机构	Guangdong Province Basic and Applied Basic Research Fund Project ; National Natural Science Foundation of China ; Major Scientific and Technological Projects of Hainan Province
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10931]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Lin, Li
作者单位	1.Zhanjiang Tianli Sea Cucumber New Technol Dev Co L, Zhanjiang 524000, Peoples R China 2.Fujian Agr & Forestry Univ, Coll Marine Sci, Fuzhou 350002, Peoples R China 3.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 4.Zhongkai Univ Agr & Engn, Coll Anim Sci & Technol, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou Key Lab Aquat Anim Dis & Waterfowl Breed, Guangzhou 510222, Peoples R China
推荐引用方式 GB/T 7714	Li, Yanan,Chen, Zongfu,Zhang, Haibin,et al. Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:12.
APA	Li, Yanan,Chen, Zongfu,Zhang, Haibin,Liu, Ruoyu,Chen, Shuichun,&Lin, Li.(2024).Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,12.
MLA	Li, Yanan,et al."Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):12.