CRISPR/Cas12a controlled chemiluminescence resonance energy transfer sensor for detection of carcinoembryonic antigen

doi:10.1016/j.snb.2023.135094

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	CRISPR/Cas12a controlled chemiluminescence resonance energy transfer sensor for detection of carcinoembryonic antigen
	Zhou, Yanmei 2,3,4,5; Xie, Shupu 4,5; Li, Mingjie 4,5; Xin, Liantao 4,5; Zhang, Xiaoru 4,5; Liu, Yi 1
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2024-02-15
卷号	401 页码:7
关键词	CRET Hemin/G-quadruplexes CRISPR/Cas12a CEA
DOI	10.1016/j.snb.2023.135094
通讯作者	Zhang, Xiaoru(zhangxr7407@126.com) ; Liu, Yi(janeliuyi@qdu.edu.cn)
英文摘要	A CRISPR-Cas12a controlled chemiluminescence resonance energy transfer (CRET) biosensor was constructed for the detection of cancer biomarker carcinoembryonic antigen (CEA). The CRET probe, a single-strand DNA (ssDNA) with hemin/G-quadruplex on one end and CdSe/ZnS quantum dots (QDs) nanosphere on the other end, was used as the substrate for the trans-cleavage of CRISPR/Cas12a system. The hemin/G-quadruplexes catalyzed the oxidation of luminol in the presence of H2O2. The generating chemiluminescence could excite the CdSe/ZnS QDs encapsulated in the nanosphere, producing CRET. When target CEA specifically identified dual aptamers on the surface of magnetic beads (MBs), the terminal sequence of Aptamer 2 triggered the hybridization chain reaction (HCR), whose product initiated the trans-cleavage activity of CRISPR/Cas12a and destroyed the CRET system. As a result, the luminescence of QDs nanosphere was declined. With HCR, CRISPR-Cas12a transcleavage, and mimic DNAzyme catalytic amplification, the biosensor gave a detection limit down to 5.6 pg/mL. Due to the application of CRISPR/Cas12a, the CRET sensor with the characteristics of easy operation and no external light source required was more suitable for point-of-care testing (POCT) detection of various targets.
资助项目	National Natural Science Foundation of China[22276103] ; Shandong Provincial Natural Science Foundation[ZR2023QH055]
WOS关键词	QUANTUM-DOTS ; CRISPR-CAS12A ; DNA
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:001132222700001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/184209]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Zhang, Xiaoru; Liu, Yi
作者单位	1.Qingdao Univ, Dept Gastroenterol, Affiliated Hosp, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 5.Qingdao Univ Sci & Technol, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, Shandong Key Lab Biochem Anal, MOE, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yanmei,Xie, Shupu,Li, Mingjie,et al. CRISPR/Cas12a controlled chemiluminescence resonance energy transfer sensor for detection of carcinoembryonic antigen[J]. SENSORS AND ACTUATORS B-CHEMICAL,2024,401:7.
APA	Zhou, Yanmei,Xie, Shupu,Li, Mingjie,Xin, Liantao,Zhang, Xiaoru,&Liu, Yi.(2024).CRISPR/Cas12a controlled chemiluminescence resonance energy transfer sensor for detection of carcinoembryonic antigen.SENSORS AND ACTUATORS B-CHEMICAL,401,7.
MLA	Zhou, Yanmei,et al."CRISPR/Cas12a controlled chemiluminescence resonance energy transfer sensor for detection of carcinoembryonic antigen".SENSORS AND ACTUATORS B-CHEMICAL 401(2024):7.