CRISPR/Cas9-mediated gene mutation of <i>EcIAG</i> leads to sex reversal in the male ridgetail white prawn <i>Exopalaemon carinicauda</i>

doi:10.3389/fendo.2023.1266641

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda
	Miao, Miao 1,2,3; Li, Shihao 1,2,4; Yuan, Jianbo 1,2,4; Liu, Peipei 1,2,3; Fang, Xiaochen 1,2,3; Zhang, Chengsong 1,2,4; Zhang, Xiaojun 1,2,4; Li, Fuhua 1,2,4,5
刊名	FRONTIERS IN ENDOCRINOLOGY
	2023-11-21
卷号	14 页码:11
关键词	insulin-like androgenic gland hormone Exopalaemon carinicauda CRISPR/Cas9 sex reversal gene editing
ISSN号	1664-2392
DOI	10.3389/fendo.2023.1266641
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	In the culture of crustaceans, most species show sexual dimorphism. Monosex culture is an effective approach to achieve high yield and economic value, especially for decapods of high value. Previous studies have developed some sex control strategies such as manual segregation, manipulation of male androgenic gland and knockdown of the male sexual differentiation switch gene encoding insulin-like androgenic gland hormone (IAG) in decapods. However, these methods could not generate hereditable changes. Genetic manipulation to achieve sex reversal individuals is absent up to now. In the present study, the gene encoding IAG (EcIAG) was identified in the ridgetail white prawn Exopalaemon carinicauda. Sequence analysis showed that EcIAG encoded conserved amino acid structure like IAGs in other decapod species. CRISPR/Cas9-mediated genome editing technology was used to knock out EcIAG. Two sgRNAs targeting the second exon of EcIAG were designed and microinjected into the prawn zygotes or the embryos at the first cleavage with commercial Cas9 protein. EcIAG in three genetic males was knocked out in both chromosome sets, which successfully generated sex reversal and phenotypic female characters. The results suggest that CRISPR/Cas9-mediated genome editing technology is an effective way to develop sex manipulation technology and contribute to monosex aquaculture in crustaceans.
资助项目	National Key R&D Program of China[2022YFD2401400] ; Earmarked fund for CARS-48 ; Taishan Scholars Program
WOS关键词	EXPRESSION ANALYSIS ; CDNA CLONING ; FEMALE ; MONOSEX ; HORMONE ; GROWTH ; IDENTIFICATION ; HOMOLOG
WOS研究方向	Endocrinology & Metabolism
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:001118980000001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/184106]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Miao, Miao,Li, Shihao,Yuan, Jianbo,et al. CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda[J]. FRONTIERS IN ENDOCRINOLOGY,2023,14:11.
APA	Miao, Miao.,Li, Shihao.,Yuan, Jianbo.,Liu, Peipei.,Fang, Xiaochen.,...&Li, Fuhua.(2023).CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda.FRONTIERS IN ENDOCRINOLOGY,14,11.
MLA	Miao, Miao,et al."CRISPR/Cas9-mediated gene mutation of EcIAG leads to sex reversal in the male ridgetail white prawn Exopalaemon carinicauda".FRONTIERS IN ENDOCRINOLOGY 14(2023):11.