Molecular and physiological responses in the ammonia transport pathways in clam<i> Cyclina</i><i> sinensis</i> exposed to chronic ammonia nitrogen

doi:10.1016/j.aqrep.2024.101952

CORC > 海洋研究所 > 中国科学院海洋研究所

	Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen
	Ni, Qian 3; Liang, Xiafei 3; Yang, Shengyi 3; Ge, Hongxing 2,3,4; Dong, Zhiguo 1,3,4
刊名	AQUACULTURE REPORTS
	2024-04-01
卷号	35 页码:9
关键词	CsRhbg GSase GDH activity Cyclina sinensis Chronic ammonia nitrogen exposure
ISSN号	2352-5134
DOI	10.1016/j.aqrep.2024.101952
通讯作者	Ge, Hongxing(hongxinggeliu@163.com) ; Dong, Zhiguo(dzg7712@163.com)
英文摘要	The clam Cyclina sinensis is characterized by good ammonia resistance. With the purpose of gaining knowledge about its ammonia resistance, we assessed the mRNA expression levels of Rhesus glucoprotein b (CsRhbg), the activities of key enzymes in the ammonia transport pathways (glutamine synthetase, GS; glutamic acid dehydrogenase, GDH) in the gill and hepatopancreas and the gill histopathological section (HE staining) of the clam exposed to total ammonia nitrogen (TAN) (6.58 mg/L) for 768 h (32d). Results revealed that the full-length cDNA sequence of CsRhbg was 1777 bp in length. The CsRhbg was highly conserved in the same genus, and the protein encoded by CsRhbg contains 10 transmembrane structures (TMHs) and one signal peptide. Exposed to ammonia nitrogen, the expression level of CsRhbg mRNA climbed sharply at 3 h post-exposure, and then it decreased and fluctuated from 192 h to 768 h. The activity of GSase in the clam exposed to ammonia nitrogen increased in the first 24 h, and reached the highest value at 96 h, and then fluctuated. The activity of GDH rose sharply and reached the highest value at 3 h, and then fluctuated. With the increase of exposure time, histological alterations in the gill became more and more clear in the disappearance of the epithelial cell boundaries. The results suggest that exposure to ammonia nitrogen can activate CsRhbg gene expression, and elevate the GSase and GDH activity in the gill of the clam as the ammonia detoxification strategy to reduce the harm of ammonia. However, prolonged exposure to ammonia nitrogen can result in the down-regulation of CsRhbg gene, dysfunction of key enzymes in the ammonia transport pathways, and damage to the structure of epithelial cells or the cellular structure in the gill of the clam. Therefore, this work will help to reveal the ammonia detoxification strategy in bivalves exposed to chronic ammonia nitrogen.
资助项目	China Agriculture Research System of MOF ; Postdoctoral Fellowship of Lianyungang[SH20211209] ; Opening Foundation of Jiangsu Key Laboratory of Marine Bioresources and Environment[JSIMR202017] ; Opening Foundation of Jiangsu Institute of Marine Resources Development[JBGS [2021] 034] ; JBGS Project of Seed Industry Revitalization in Jiangsu Province[CARS-49] ; China Agriculture Research System of MARA ; Priority Academic Program Development of Jiangsu ; [LYG20210008]
WOS关键词	RAINBOW-TROUT ; GLUTAMINE-SYNTHETASE ; RHESUS GLYCOPROTEINS ; SUBLETHAL AMMONIA ; EXPRESSION ; BRAIN ; INHIBITION ; METABOLISM ; EXCRETION ; FAMILY
WOS研究方向	Fisheries
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001173451900001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/184577]
专题	中国科学院海洋研究所
通讯作者	Ge, Hongxing; Dong, Zhiguo
作者单位	1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 3.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222005, Jiangsu, Peoples R China 4.Jiangsu Marine Resources Dev Inst, Lianyungang 222005, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Ni, Qian,Liang, Xiafei,Yang, Shengyi,et al. Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen[J]. AQUACULTURE REPORTS,2024,35:9.
APA	Ni, Qian,Liang, Xiafei,Yang, Shengyi,Ge, Hongxing,&Dong, Zhiguo.(2024).Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen.AQUACULTURE REPORTS,35,9.
MLA	Ni, Qian,et al."Molecular and physiological responses in the ammonia transport pathways in clam Cyclina sinensis exposed to chronic ammonia nitrogen".AQUACULTURE REPORTS 35(2024):9.