Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction

doi:10.3390/jmse12010061

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction
	Xu, Peikuan 2; Yang, Ming 1; He, Lisheng 1; Zhang, Hongxi 1; Gao, Zhaoming 1; Jiang, Yuelu 2; Wang, Yong 2; Antonarakou, Assimina
刊名	JOURNAL OF MARINE SCIENCE AND ENGINEERING
	2024
卷号	12 期号:1 页码:12
关键词	eukaryotic microbe MISNAC Retaria South China Sea Niskin
DOI	10.3390/jmse12010061
英文摘要	Deep-sea eukaryotic microorganisms play a vital role in degrading organic matter and geochemically cycling elements in the deep ocean. However, the impact of sampling methods on detection of these microorganisms under high hydrostatic pressure remains uncertain. In this study, we compared a traditional water sampling method using a Niskin bottle, an in situ microbial filtration and fixation method (ISMIFF), and a multiple in situ nucleic acid collection (MISNAC) method to exhibit differences in the community structures that were collected at similar to 590-3100 m in the South China Sea (SCS). The classification and biodiversity indices of 18S rDNA Illumina sequencing reads from the V9 variation region revealed higher diversity for MISNAC DNA absorption column samples compared to others. Importantly, the relative abundance of Ciliophora (19.49%), Copepoda (23.31%), and Diplonemea (10.67%) was higher in MISNAC adsorption column samples, while Retaria (48.86%) were dominant in the MISNAC membrane samples. This indicates that MISNAC columns might collect more DNA in situ for the naked protists, while Retaria with a carbonate shell were more likely retained on the membrane. In conclusion, MISNAC is an effective method for DNA collection of deep-sea eukaryotic microorganisms and provides valuable materials for studying deep-sea microbial ecosystems.
资助项目	Hainan Provincial Natural Science Foundation of China ; Supercomputer Center of Sanya University
WOS关键词	MICROBIAL EUKARYOTES ; SEQUENCES ; TOOL
WOS研究方向	Engineering ; Oceanography
语种	英语
出版者	MDPI
WOS记录号	WOS:001152719100001
资助机构	Hainan Provincial Natural Science Foundation of China ; Supercomputer Center of Sanya University
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10845]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
通讯作者	Jiang, Yuelu; Wang, Yong
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Tsinghua Univ, Inst Ocean Engn, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Xu, Peikuan,Yang, Ming,He, Lisheng,et al. Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2024,12(1):12.
APA	Xu, Peikuan.,Yang, Ming.,He, Lisheng.,Zhang, Hongxi.,Gao, Zhaoming.,...&Antonarakou, Assimina.(2024).Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction.JOURNAL OF MARINE SCIENCE AND ENGINEERING,12(1),12.
MLA	Xu, Peikuan,et al."Discovery of Prevalent Ciliophora, Discoba, and Copepoda Protists in Deep Sea Water by In Situ Nucleotide Extraction".JOURNAL OF MARINE SCIENCE AND ENGINEERING 12.1(2024):12.